脐带间充质干细胞对乳腺癌细胞系MCF-7裸鼠移植瘤生长的影响  

Effect of umbilical cord mesenchymal stem cells on the growth of human breast cancer MCF-7 xenograft in a nude mouse

作　　者：韩丽鑫[1] 韩之波[1] 耿洁[2] 王斌[2] 严淑玲[2] 毛爱斌 韩忠朝[1,2] 

机构地区：[1]中国医学科学院北京协和医学院血液病医院血液学研究所,实验血液学国家重点实验室,天津市300020 [2]细胞产品国家工程研究中心,天津市300457

出　　处：《中国组织工程研究》2015年第19期2986-2992,共7页Chinese Journal of Tissue Engineering Research

基　　金：国家科技支撑计划(2013BAI01B09)~~

摘　　要：背景:已有临床前和临床研究资料表明,间充质干细胞预防和治疗异基因造血干细胞移植后移植物抗宿主病、自身免疫性疾病以及糖尿病等具有显著疗效。但间充质干细胞植入后是否会促进体内肿瘤生长是其临床应用安全性的一个重要体现,目前这部分内容研究尚少。目的:观察脐带间充质干细胞对人乳腺癌细胞MCF-7荷瘤裸鼠肿瘤生长的影响。方法:随机抽取BALB/c裸鼠8只作为正常对照组,随机抽取乳腺癌细胞系MCF-7裸鼠分为4组,每组8只,分别为模型对照组、低剂量组、中剂量组和高剂量组。3个剂量组尾静脉注射脐带来源的间充质干细胞,分别为4×104、2×105和1×106个,共给予2次,间隔2周;正常对照组和模型对照组注射生理盐水。实验观察周期为6周。结果与结论:模型对照组肿瘤体积呈持续增长趋势,观察结束后各剂量组与同期模型对照组动物相比,脐带间充质干细胞有抑制肿瘤体积及质量的趋势,但差异无显著性意义,病理组织学结果显示中高剂量组各有1只动物肺脏可见转移瘤。结果表明脐带间充质干细胞不促进MCF-7裸鼠肿瘤的生长,但可促进MCF-7裸鼠转移瘤的形成。BACKGROUND： Mesenchymal stem cells reveal notable therapeutic effects on diabetes, graft-versus-host disease and autoimmune diseases after allogeneic hematopoietic stem cell transplantation. Clinical safety is crucial for the clinical application of mesenchymal stem cells, and whether transplated mesenchymal stem cells can promote tumor growth in vivo or not is an important manifestation; however, fewer studies have been reported on this field. OBJECTIVE： To observe the effect of umbilical cord mesenchymal stem cells on growth of human breast cancer MCF-7 xenograft in a nude mouse in vivo. METHODS： Eight BALB/c nude mice were randomly selected as controls, and nude mice with human breast cancer MCF-7 xenograft were randomly divided into four groups with eight mice in each group： model control group, low-dose group, middle-dose group and high-dose group. Three different doses of umbilical cordmesenchymal stern cells （4×10^4、2×10^5 and 1×10^6/mice） were given twice in the latter three groups, respectively, with an interval of 2 weeks. Healthy nude mice and MCF-7 tumor-burdened mice without transplantation were given normal saline. The experimental observation period was 6 weeks. RESULTS AND CONCLUSION： The tumor size exhibited an increasing trend in the model control group, Umbilical cord mesenchymal stem cells could inhibit tumor growth, but there was no difference among different groups. Histopathological analysis showed that one mouse had lung metastases in either high-dose or middle dose-group. These findings indicate that umbilical cord mesenchymal stem cells cannot promote the tumor growth in a MCF-7 xenograft nude mouse, but they can accelerate the tumor metastases.

关 键 词：脐带 间质干细胞移植 乳腺肿瘤 小鼠 裸 干细胞 脐带脐血干细胞 脐带间充质干细胞 MCF-7 裸鼠 转移瘤 

分 类 号：R394.2[医药卫生—医学遗传学]