大鼠肝癌诱导过程中肝癌干细胞标志及炎症因子的动态变化  

Dynamic changes of liver cancer stem cell markers and inflammatory factors during the induction of liver cancer in rats

作　　者：郑飞[1] 周文平[1] 张巍[1] 赵正维[2] 

机构地区：[1]解放军沈阳军区总医院,辽宁省沈阳市110016 [2]解放军第四军医大学唐都医院,陕西省西安市710038

出　　处：《中国组织工程研究》2015年第19期3005-3009,共5页Chinese Journal of Tissue Engineering Research

摘　　要：背景:近年来已有研究者从肝癌组织和细胞系中发现了CD133、乙醛脱氢酶(ALDH)、CD90、CD44、Epc AM、CD13、OV6、K19、c-kit、ABCG2等多种肝癌干细胞的标志物,其中CD家族的CD133、CD90、CD44等被认为与肝癌的复发和转移密切相关。目的:探讨大鼠肝癌诱导过程中肝癌干细胞标志及炎症因子的动态变化及两者相关性。方法:应用二乙基亚硝胺(DEN)溶液饲养SD大鼠24周诱导大鼠肝癌模型,并设立普通水喂养的健康对照组。结果与结论:免疫组织化学检测显示,模型组肝癌诱导过程中Kupffer细胞相关ED2表达呈现出逐渐增多的情况,与健康对照组比较,模型组ED2在诱癌第12,16,20,24周的表达均显著升(P<0.05)。定量PCR检测显示,肝癌诱导过程中CD90呈逐渐上升趋势(P<0.05),较之健康肝脏组织,肝癌组织中CD90上升更明显(P<0.05);CD133呈现出一定升高趋势,但经单因素方差分析无统计学意义(P>0.05);在诱癌过程中其他肝癌干细胞标志物未出现明显改变(P>0.05)。肝癌诱导过程中,肿瘤坏死因子α、转化生长因子β、MCP-1及白细胞介素6均明显上升(P<0.05),较之健康肝脏组织,肝癌组织中转化生长因子β、MCP-1、白细胞介素6表达显著偏高(P<0.05),其余炎症因子在诱癌过程中则未出现明显改变(P>0.05)。经Pearson相关性分析,MCP-1、转化生长因子β、白细胞介素6与CD90的表达之间呈显著正相关(P<0.05)。表明Kupffer细胞所释放的部分炎症因子与肝癌干细胞标志之间存在一定的相关性,Kupffer细胞可促进肝癌的发生。BACKGROUND： Many liver cancer stem cell markers have been found in liver cancer tissues and cell lines such as CD133, acetaldehyde dehydregenase （ALDH）, CD90, CD44, EpcAM, CD13, OV6, K19, c-kit and ABCG2. Of them, CD133, CD90 and CD44 have been shown to be strongly associated with the recurrence and metastasis of liver cancer. OBJECTIVE： To explore the dynamic changes of liver cancer stem cell markers and inflammatory factors during the induction of liver cancer in rats and their correlation. METHODS： Diethyl nitresamine solution was given to Sprague-Dawley rats for 24 hours to induce rat models of liver cancer. Rats that were given common water were considered as the healthy control group. RESULTS AND CONCLUSION： Immunohistochemical staining revealed that Kupffer calls-related ED2 expression showed a gradual increase in the model group. Compared with the healthy control group, ED2 expression was significantly higher at 12, 16, 20 and 24 weeks after induction in the model group （P 〈 0.05）.Quantitative PCR demonstrated that CD90 showed a gradually increased trend during induction （P 〈 0.05）. Compared with healthy tissue, CD90 increased significantly in the liver cancer tissue （P 〈 0.05）. CD133 showed an increased trend, but one-way analysis of variance did not show significant differences （P 〉 0.05）. During induction, no significant change was found in other liver cancer stem cell markers （P 〉 0.05）. During the induction, tumor necrosis factor o, transforming growth factor β, MCP-1 and interleukin-6 expression levels were significantly increased （P 〈 0.05）. Compared with healthy tissue, transforming growth factor Ⅱ, MCP-1 and interleukin-6 expression levels were significantly higher in the liver cancer tissue （P 〈 0.05）. Other inflammatory factors did not exhibit significant alterations during the induction （P 〉 0.05）. Pearson correlation analysis demonstrated that MCP-1, transforming growth factor β and interleukin-6 expression levels were significan

关 键 词：肿瘤干细胞 肝肿瘤 肿瘤标记 生物学 干细胞 肝癌干细胞标志 肝癌 炎症因子 动物实验 相关性 

分 类 号：R394.2[医药卫生—医学遗传学]