不同生长因子对脂肪干细胞生物学行为的影响  

Effects of different growth factors on biological behaviors of adipose tissue-derived stem cells

作　　者：高洁[1] 王明国[2] 杨帅[1] 李雪[1] 杨世茂[2] 李秀梅[3] 刘金盼[3] 

机构地区：[1]潍坊医学院口腔医学院,山东省潍坊市261053 [2]济南市中心医院口腔科,山东省济南市250013 [3]济南市市中区人民医院口腔科,山东省济南市250002

出　　处：《中国组织工程研究》2015年第19期3010-3016,共7页Chinese Journal of Tissue Engineering Research

基　　金：山东省科技发展项目(2014GSF11804)~~

摘　　要：背景:骨量不足限制了口腔种植修复的广泛应用,如何促进干细胞的迁移、黏附和增殖进而促进内源骨再生成为研究的关键点。目的:观察不同生长因子对体外培养兔脂肪干细胞迁移、黏附和增殖的影响,筛选出最佳的组合因子。方法:无菌切除兔腹股沟处的白色脂肪组织,采用酶消化法培养脂肪干细胞,取第3代细胞分为5组进行干预,分别为转化生长因子β1+血小板源性生长因子AB组(组1),血小板源性生长因子AB+血管内皮生长因子组(组2),转化生长因子β1+血管内皮生长因子组(组3),转化生长因子β1+血小板源性生长因子AB+血管内皮生长因子组(组4)和空白对照组。采用Transwell小室法检测细胞的迁移能力,黏附实验检测细胞的黏附能力,CCK-8法检测细胞的增殖能力。结果与结论:Transwell实验显示,4组间迁移细胞数差异有显著性意义(P<0.05),其中,转化生长因子β1(2μg/L)+血小板源性生长因子AB(10μg/L)+血管内皮生长因子(10μg/L)组(组4)迁移细胞数最多,显著促进了脂肪干细胞的迁移。黏附实验结果显示,4组间黏附细胞数差异有显著性意义(P<0.05),其中转化生长因子β1+血管内皮生长因子组(组3)黏附细胞数最多,显著促进了脂肪干细胞的黏附。CCK-8结果显示,在不同的时间点(1,3,5,7 d),各因子组合组吸光度值较对照组均显著增高(P<0.05),其中血小板源性生长因子AB+血管内皮生长因子组(组2)递增的吸光度值最大,显著促进了兔脂肪干细胞的增殖。BACKGROUND： Bone deficiency restricts the extensive use of oral implant restoration. How to promote stem cell migration, adhesion and proliferation so as to promote endogenous bone regeneration becomes the key of research. OBJECTIVE： To observe the effects of different growth factors on the migration, adhesion and proliferation of rabbit adipose tissue-derived stem cells cultured in vitro and to screen the optimal combined factors. METHODS： The inguinal adipose tissues of rabbits were cut under aseptic conditions and primary adipose tissue-derived stem cells were cultured by enzyme digestion method. Passage 3 cells were randomly divided intofive groups： transforming growth factor i31＋platelet-derived growth factor AB group （group 1）, platelet-derived growth factor AB＋vascular endothelial growth factor group （group 2）, transforming growth factor 131 ＋vascular endothelial growth factor group （group 3）, transforming growth factor 131 ＋platelet-derived growth factor AB＋vascular endothelial growth factor group （group 4）, and blank control group. In vitro cell migration ability was examined by Transwell chamber. Cell proliferation and adhesion abilities were examined by cell counting kit-8 and cell adhesion assay, respectively. RESULTS AND CONCLUSION： Results from the Transwell test showed that the migration number of rabbit adipose tissue-derived stem cells showed significant differences among the former four groups （P 〈 0.05）, which was highest in the group 4 that significantly promoted the migration of adipose tissue-derived stem cells. In the cell adhesion assay, there were significant differences in the number of adherent cells among the former four groups （P 〈 0.05）, and the group 3 showed the most adherent cells that dramatically improved the adhesion of adipose tissue-derived stem cells. Compared with the blank control group, the absorbance values in the groups 1-4 were significantly higher at days 1,3, 5, 7 （P 〈 0.05）, and the group 2 showed the highest abs

关 键 词：脂肪组织 干细胞 细胞运动 细胞粘附 细胞增殖 转化生长因子β1 血小板源性生长因子 血管内皮生长因子类 脂肪干细胞 转化生长因子 血小板源性生长因子AB 血管内皮生长因子 

分 类 号：R394.2[医药卫生—医学遗传学]