益髓生血颗粒对血红蛋白H病患者抗氧化损伤和细胞因子水平的影响  

Level of Anti-oxidative Damage and Cytokine in Patients of Hb H Disease Treated by Yisui Shengxue Granule

作　　者：褚娜利[1] 吴志奎[1] 张新华[2] 王文娟[3] 程艳玲[1] 方素萍[1] 

机构地区：[1]中国中医科学院广安门医院,北京100053 [2]中国人民解放军第303医院,南京530021 [3]首都医科大学,北京100069

出　　处：《医学研究杂志》2015年第6期35-38,共4页Journal of Medical Research

基　　金：国家自然科学基金资助项目(81173167);国家重点基础研究发展计划("973"计划)项目(2010CB530406)

摘　　要：目的 研究益髓生血颗粒对血红蛋白H病患者抗氧化损伤和造血相关细胞因子水平的影响.方法 采用安慰剂平行对照实验方法,选择30例血红蛋白H病患者,随机分为治疗组和对照组,每组15例,治疗组给予益髓生血颗粒进行治疗,对照组给予安慰剂进行治疗,疗程均为3个月.动态监测治疗前后患者Hb、RBC、HbH的水平;检测治疗前后血清及红细胞氧化与抗氧化损伤指标（SOD、MDA、GSH-PX）和细胞因子（SCF、GM-CSF、IL-3）活性.结果 自益髓生血颗粒治疗1～3个月,治疗组患者疗效性血液指标Hb水平与治疗前相比显著升高（P ＜0.01,P＜0.05）;RBC疗后1、3个月水平与疗前相比显著升高（P＜0.05）;Ret水平治疗后有升高的趋势.对照组经安慰剂治疗后血液指标Hb、RBC、Ret水平与疗前相比均无明显改善.经益髓生血颗粒治疗3个月后,治疗组患者红细胞SOD、GSH-Px水平与疗前相比显著升高（P＜0.01）;MDA水平与疗前相比,显著降低（P＜0.01）;血清SOD水平与疗前相比显著升高（P＜0.01）;对照组各指标水平均无明显变化.经益髓生血颗粒治疗3个月后,治疗组患者SCF水平与疗前相比显著升高（P＜0.05）;GM-CSF、IL-3水平与疗前相比无明显变化;对照组各指标水平均无明显变化.结论 益髓生血颗粒治疗地中海贫血有良好的临床疗效,能显著改善患者血液参数水平.益髓生血颗粒的疗效生物学机制之一与提高HbH病患者抗氧化损伤能力和造血正调控细胞因子SCF活性有关.Objective To study the influence of Yisui Shengxue granule on the levels of anti - oxidative damage and cytokine of pa- tients with HbH disease. Methods A randomized single - blinded trial was designed. Thirty patients with HbH disease were divided in- to two groups ： 15 patients in YSSXG - treated group and 15 in placebo parallel control group. The patients in the two groups were assigned to receive either YSSXG or placebo for three months. The blood parameters [ hemoglobin（Hb） , red blood cell （RBC） and reticulocytes （Ret） ] were detected dynamically. We also detected the SOD, MDA and GSH - PX from blood serum as well as red blood cell and the ac- tivity of SCF, GM - CSF and IL - 3 from blood serum. Results After the treatment of Yisui Shengxue granule,the levels of blood parame- ter Hb of patients in the treatment group from the first to the end of months were still significantly higher than those of pre - treatment （P 〈 0.01 or P 〈 0.05 ） and the levels of RBC at the first and the third month were significantly higher than those of pre - treatment ;the level of Ret had a rising tendency. The measurements of blood parameters in the placebo group had no statistically changing compared with those of pre - treatment. The activities of erythrocytic SOD and GSH - Px and the serum SOD were significantly higher than those of pre - treat- ment （ P 〈 0.01 ） and the activity of erythrocytic MDA was significantly lower than that of pre - treatment（ P 〈 0. 01 ）. The indicators of an- ti - oxidative damage of the placebo group were no remarkable changing. The level of SCF was significantly higer than that of pre - treat- ment （ P 〈 0.05 ） and the levels of GM - CSF and IL - 3 had no significant difference with those of pre - treatment in the treatment group as well as in the placebo group. Conclusion Yisui Shengxue granule has a positive effect for the patients with HbH disease and dramatic- ally improves the levels of blood parameters. One of biological mechanisms of curative effeca

关 键 词：血红蛋白H病 益髓生血颗粒 血液参数 抗氧化损伤 细胞因子 

分 类 号：R552[医药卫生—血液循环系统疾病]