口腔扁平苔藓患者外周血Th1/Th2型细胞因子表达及意义  

Expression and significance of Th1/Th2 cytokines in peripheral blood of patients with oral lichen planus

作　　者：罗亮[1] 王冬平[1] 蔡扬[1] 张洛[2] 

机构地区：[1]贵阳医学院附属医院口腔内科,贵阳550004 [2]贵州省人民医院口腔科,贵阳550002

出　　处：《重庆医科大学学报》2015年第5期743-746,共4页Journal of Chongqing Medical University

基　　金：国家自然科学基金资助项目(编号:81460104);贵州省优秀科技教育人才省长资金资助项目(编号:黔省专合字(2009)38号)

摘　　要：目的：了解口腔扁平苔藓（oral lichen planus,OLP）患者外周血中Th1、Th2型细胞因子表达水平,探讨Th1/Th2平衡在OLP发病中的作用及意义。方法：采用流式细胞术（cytometric bead array,CBA）检测51例OLP患者（网纹型23例,充血糜烂型28例）Th1型细胞因子（TNF-α、IFN-γ、IL-2）及Th2型细胞因子（IL-4、IL-6、IL-10）表达水平,并分析其与OLP临床特征相关性。结果：1OLP患者血清中Th2型细胞因子的IL-6与IL-10的表达水平明显高于正常对照组（P〈0.05）,但Th1型细胞因子TNF-α、IFN-γ、IL-2及Th2型细胞因子IL-4表达水平与正常组差异无统计学意义（P〉0.05）;2Th2型细胞因子IL-6的表达水平与OLP临床类型、临床体征、临床症状均成正相关,IL-10的表达水平与OLP临床类型、临床体征均成正相关（相关系数0.285~0.364;P〈0.05）。结论：OLP患者外周血中Th1型细胞因子的表达水平与正常对照组Th1型细胞因子表达水平差异无明显改变,而Th2型细胞因子升高,表现为Th2占优势的免疫应答;Th2型细胞因子IL-6、IL-10表达与OLP患者病情严重程度呈正相关,对OLP免疫治疗有一定指导意义。Objective：To investigate the expression of Th1/Th2 cytokines in peripheral blood of patients with oral lichen planus（OLP）and to discuss the role and significance of Th1/Th2 cytokines in the pathogenesis of OLP. Methods：Totally 51 cases of OLP with different clinical characters（including 23 cases of reticular OLP and 28 cases of hyperaemia erosion OLP） were enrolled. Cytometric bead array was employed to detect the level of type 1 cytokines（TNF-α,INF-γ,IL-2）and type 2 cytokines（IL-4,IL-6,IL-10）of T helper cells in serum of patients with OLP. The relationship between the levels of these cytokines and the clinical characteristics of patients with OLP was analyzed. Results：Expression levels of IL-6 and IL-10 in the peripheral blood of patients with OLP were statistically increased compared with those of controls（P〈0.05）,but no statistical difference was observed in level of Th1 cytokines（TNF-α,IFN-γ,IL-2）and IL-4 between OLP patients and controls（P〉0.05）. Expression levels of IL-6 and IL-10 were positively correlated with clinical characters（r：0.285-0.364;P〈0.05）. Conclusion：Th1 cytokines remain unchanged and Th2 cytokines increase in OLP patients,showing the advantage of Th2 immune response. Levels of IL-6 and IL-10 are positively correlated with disease severity,which may be helpful for OLP immunotherapy.

关 键 词：口腔扁平苔藓 辅助性T细胞 细胞因子微球检测技术 

分 类 号：R781.59[医药卫生—口腔医学]