富含n-3多不饱和脂肪酸的肠外营养对脓毒症大鼠T淋巴细胞亚群和炎性因子的影响  

Effects of fish oil-supplemented parenteral nutrition on plasma cytokines and peripheral blood T lymphocyte subsets in rats with abdominal sepsis

作　　者：李晓龙[1] 张宪祥[1] 杨恩钦 张楠阳[2] 曹守根[1] 周岩冰[1] 

机构地区：[1]青岛大学附属医院普通外科,山东青岛266003 [2]青岛大学附属医院医学动物实验室,山东青岛266003

出　　处：《肠外与肠内营养》2015年第3期180-183,共4页Parenteral & Enteral Nutrition

基　　金：国家自然科学基金资助项目(81270449);山东省自然科学基金资助项目(ZR2012HM046);青岛市民生科技计划项目(14-2-3-5-nsh);青岛市科技计划项目(13-1-4-220-jch)

摘　　要：目的:探讨富含n-3多不饱和脂肪酸(PUFA)的肠外营养(PN)对脓毒症大鼠外周血T淋巴细胞亚群和血清炎性因子的影响。方法:将36只大鼠随机分为四组,颈静脉置管术2 d后行盲肠结扎穿孔术或假手术,1对照组:给予自由饮食;2NS组:给予等渗盐水输注+自由饮食;3SO组:给予富含豆油的标准肠外营养(PN)液;4FO组:给予富含PUFA的PN液。脓毒症5 d后,检测IL-6、IL-10和T细胞亚群等。结果:脓毒症5 d后,FO组大鼠外周血CD3+、CD4+、CD4+/CD8+与NS组和SO组比有所升高,但较对照组大鼠下降;与NS组大鼠比,FO组血清IL-6、IL-10、外周血Tregs比例有所下降。结论:应用富含n-3 PUFA的PN可降低脓毒症大鼠血清炎性因子和调控外周血T淋巴细胞亚群。Objective： To investigate the effect of n-3PUFA on serum cytokines and peripheral blood T lymphocyte subsets in rats with abdominal sepsis. Methods： Thirty-six adult male SpragueDawley rats were divided into four groups randomly. Two days after central venous catheterization,rats in3 sepsis groups were subjected to cecal ligation and puncture to produce abdominal sepsis models. Rats were assigned to receive normal saline（ NS group） or total parenteral nutrition（ TPN） containing a standard soybean oil emulsion（ SO group） or FO-supplemented TPN（ FO group） at the onset of sepsis for 5 days. Rats in the control group underwent sham operation and approriate control treatment. Then the serum concentrations of IL-6,IL-10 and distribution of T lymphocyte subsets in peripheral blood were measured. Results： Compared with normal saline and TPN without fish oil,FO-supplemented TPN could increase the proportions of CD3＋,CD4＋and CD4＋/ CD8＋in peripheral blood,which were still lower than that in the control group. Plasma IL-6 as well as IL-10 and Tregs in peripheral blood in FO group were lower than that in NS group. Conclusion： FO-supplemented TPN can decrease the serum concentrations of cytokines and modulate peripheral blood T lymphocyte subsets in rats with abdominal sepsis.

关 键 词：N-3多不饱和脂肪酸 肠外营养 脓毒症 炎性因子 免疫功能 

分 类 号：R459.7[医药卫生—急诊医学]