血清胸苷激酶1在肿瘤患者中的表达及临床意义  

The expression and clinical significance of serum thymidine kinase 1 in cancer patients

作　　者：朱艳哲[1] 马泰[1] 张从军[1] 孙国平[1] 

机构地区：[1]安徽医科大学第一附属医院肿瘤内科,合肥230022

出　　处：《安徽医科大学学报》2015年第7期1012-1015,共4页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:81272739)

摘　　要：目的探讨血清胸苷激酶1(TK1)检测作为肿瘤细胞增殖标志物对肿瘤早期预测的意义,探讨血清TK1的含量与肿瘤病种、复发转移、临床分期及临床治疗效果评估的关系。方法应用酶免疫点印迹化学发光法测定144例肿瘤患者(肿瘤患者组)和67例健康对照者(对照组)血清TK1的水平,分析血清TK1与肿瘤患者各生物学行为参数的关系。结果肿瘤患者组和对照组在血清TK1水平及阳性表达率间的差异均有统计学意义(P<0.01),且TK1浓度与年龄呈正相关性(P<0.01)。肿瘤患者TK1的表达在不同病种之间的差异有统计学意义(P<0.05),有复发或转移的患者血清TK1阳性表达率与无复发或转移的患者比较差异有统计学意义(P<0.01),大肠癌及肺癌的Ⅰ期、Ⅱ期、Ⅲ期、Ⅳ期,各组血清TK1阳性表达率差异有统计学意义(P<0.01)。大肠癌患者治疗后不同临床疗效(完全缓解,部分缓解,疾病进展)的患者血清TK1水平的差异有统计学意义(P<0.01)。结论 TK1可用于对恶性肿瘤的早期筛查。血清TK1检测可作为临床上对肿瘤增殖程度及复发监测的手段之一。Objective To evaluate the use of serum thymidine kinase 1 （ TK1 ） as a proliferating tumor marker for early tumor detection. To investigate possible correlations between TK1 levels and categories, recrudescent or me-tastasizes , clinical staging levels and curative effect. Methods Using enhanced chemo-luminescence assay to de-tect the serum TK1 levels of 144 cancer patients and 67 healthy controls. Analyzing the relationship between the bi-ological behavior of cancer parameters and the TK1 levels. Results The concentration of TK1 in cancer patients was significantly higher compared to the control group （ P〈0. 01 ） . There exists significant difference in both TK1 concentrations and expression between cancer patients and the control group （ P〈0. 01 ） . The positive rate of TK1 expression was found to increase as age increased（P〈0. 01）. There exists significant difference in TK1 expression between different categories （ P〈0. 05 ） . There was also significant difference in TK1 expression between patients with recrudescent or metastasizes and patients without recrudescent or metastasizes （ P 〈0. 01 ） . The significant difference in TK1 expression was found between different clinical staging （Ⅰ,Ⅱ,Ⅲ,Ⅳ） of colorectal and lung cancers （ P〈0. 01 ） . There exists significant difference in TK1 concentration between untreated and post-treated cancer patients. The significant difference of concentrations of TK1 was found in routine clinical setting therapy effects （CR/PR/PD） in post-treated cancer patients （P〈0. 01）. Conclusion TK1 can predict the risk for devel-opment of neoplasia related diseases in its early stages. Concentration levels of TK1 can monitor tumor growth as well as predict the likelihood of recurrence in cancer patients.

关 键 词：血清胸苷激酶1 肿瘤标志物 酶免疫点印迹化学发光法 治疗 

分 类 号：R730.4[医药卫生—肿瘤]