加减一阴煎改善去势大鼠内皮功能紊乱  

Effect of Jia-jian-yi-yin decoction on endothelial dysfunction in ovariectomized female rats

作　　者：徐静婷[1] 曹娅麟 郑文晖[1] 张亚星[1] 王玲[1] 王庭槐[1] 

机构地区：[1]中山大学中山医学院生理教研室,广东广州510080

出　　处：《中国病理生理杂志》2015年第6期1008-1013,共6页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.81172525);广东省科技厅项目(No.2012A032500002)

摘　　要：目的:探讨加减一阴煎对去势大鼠内皮功能紊乱的影响。方法:采用血管张力仪测定SD大鼠内皮依赖性舒张功能,扫描电镜观察胸主动脉内皮细胞形态,Western blotting技术检测内皮脂肪酶的表达,HE染色观察大鼠子宫内膜的形态。结果:加减一阴煎逆转了去势大鼠内皮依赖性舒张功能的降低;加减一阴煎减少了去势大鼠内皮细胞脂质的堆积,降低了去势大鼠内皮脂肪酶的表达水平;加减一阴煎对去势大鼠子宫湿重的影响不大。结论:加减一阴煎可改善去势大鼠血管内皮依赖性舒张功能,维持内皮细胞形态的完整。AIM：To investigate the effect of Jia-jian-yi-yin decoction on endothelium-dysfunction in ovariecto-mized rats.METHODS：The ovariectomized rats were treated with Jia-jian-yi-yin decoction or turbid liquid of estradiol va-lerate for 8 weeks.The vascular ring tension was measured.Scanning electron microscopy and Western blotting were ap-plied to assess the function of endothelium-dependent dilation, aortic endothelial morphology and the expression of endothe-lial lipase.The pathologic sections were prepared to observe the effect of Jia-jian-yi-yin decoction on the uterus.RE-SULTS：In ovariectomized rats, the decrease in endothelium-dependent relaxation to acetylcholine （ ACh） was reversed to normal level, the endothelial morphology returned to normal without lipid accumulation and the endothelial lipase expression was decreased by Jia-jian-yi-yin decoction.Furthermore, no obvious change of the wet weight of uterine between the ovari-ectomized rats with or without Jia-jian-yi-yin decoction treatment was observed.CONCLUSION：Jia-jian-yi-yin decoction may have protective effects on endothelium-dependent vasodilation and aortic endothelial morphology in estrogen-deficient animals.

关 键 词：加减一阴煎 戊酸雌二醇 内皮依赖性舒张 内皮脂肪酶 

分 类 号：R363[医药卫生—病理学] R289.51[医药卫生—基础医学]