纳米金逆转人肝癌耐药细胞HepG2/ADM耐药性的实验研究  

Effect of gold nanoparticles on reversing adriamycin resistance of human hepatocellular carcinoma HepG2/ADM cells

作　　者：邵明涛[1] 潘运龙[1,2] 覃莉[3] 巫青[1] 丁晖[1] 赵晓旭[2] 

机构地区：[1]暨南大学附属第一医院胃肠外科,广东广州510632 [2]暨南大学附属第一医院体部伽玛刀中心,广东广州510632 [3]暨南大学医学院组织胚胎学教研室,广东广州510632

出　　处：《中国病理生理杂志》2015年第6期1014-1018,共5页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.81472849);广东省自然科学基金资助项目(No.2014A030313383);暨南大学第一临床医学院科研培育基金资助项目(No.511005004)

摘　　要：目的:探讨纳米金(gold nanoparticles,GNPs)对人肝癌阿霉素(adriamycin,ADM)耐药细胞株Hep G2/ADM的阿霉素耐药性的逆转作用及其可能机制。方法:MTT比色法检测GNPs对人肝癌细胞株Hep G2及其耐药细胞株Hep G2/ADM对不同浓度ADM耐药性的影响;流式细胞术检测GNPs处理后ADM(2 mg/L)对Hep G2/ADM细胞凋亡的影响;紫外分光光度计检测GNPs处理后Hep G2/ADM细胞内ADM的药物浓度;谷胱甘肽(GSH)检测试剂盒(DTNB法)检测GNPs处理后Hep G2/ADM细胞GSH的含量。结果:GNPs处理前Hep G2和Hep G2/ADM细胞对不同浓度ADM的半数抑制浓度分别为:(9.16±2.03)mg/L、(29.46±1.73)mg/L,耐药倍数为3.22;GNPs处理后Hep G2/ADM细胞对不同浓度ADM的半数抑制浓度为(15.18±0.85)mg/L,逆转指数为1.95。GNPs+ADM组Hep G2/ADM的细胞凋亡率明显高于单独ADM组(P<0.05)。Hep G2/ADM组细胞内的ADM含量低于Hep G2组细胞内的ADM含量(P<0.01);GNPs处理后Hep G2/ADM细胞内的ADM含量较处理前明显增加(P<0.01)。Hep G2/ADM组细胞内GSH含量高于Hep G2组(P<0.01);GNPs处理后Hep G2/ADM细胞内的GSH含量较处理前明显降低(P<0.05)。结论:纳米金具有逆转耐药肝癌细胞对阿霉素耐药性的作用,并且可以降低细胞内GSH含量及增加胞内化疗药物浓度。AIM：To investigate whether gold nanoparticles （GNPs） reverses adriamycin （ADM）, resistance of human hepatocellular carcinoma drug-resistant cell line HepG2/ADM and to explore the potential mechanism.METH-ODS：The sensitivities of HepG2 cells and HepG2/ADM cells to ADM were tested by MTT assay before and after GNPs pretreatment.The apoptotic rate was examined by flow cytometry.The concentration of ADM in HepG2/ADM or HepG2 cells was determined by ultraviolet-visible spectrophotometer .The content of glutathione （ GSH ） in HepG2/ADM or HepG2 cells by DTNB method.RESULTS：The half maximal inhibitory concentrations （ IC50 ） of ADM for HepG2/ADM cells were（29.46 ±1.73） mg/L and （15.18 ±0.85） mg/L before and after GNPs pretreatment,respectively.The IC50 of ADM for HepG2 cells was （9.16 ±2.03） mg/L before pretreatment.The apoptotic rate in GNPs＋ADM group was higher than that in ADM group （ P〈0.05 ） .The concentration of ADM in HepG2/ADM group was lower than that in HepG2 group （P〈0.01）.After GNPs pretreatment, the concentration of ADM in HepG2/ADM cells was higher than that before pretreatment.The content of GSH in HepG2/ADM group was higher than that in HepG2 group （P〈0.01）.After GNPs pretreatment, the content of GSH in the HepG2/ADM cells was lower than that before pretreatment.CONCLUSION：Gold nanoparticles can reverse ADM resistance of human hepatocellular carcinoma drug-resistant cell line HepG2/ADM, 〈br〉 reduce the content of GSH and increase the concentration of ADM in HepG2/ADM cells.

关 键 词：纳米金 阿霉素 谷胱甘肽 耐药性 肝细胞癌 

分 类 号：R[医药卫生]