母体肢体缺血预处理对宫内窘迫胎鼠复氧后海马神经元线粒体结构和功能的影响  

Effects of maternal limb ischemic preconditioning on structural and functional changes of mitochondria in fetal hippocampal neurons induced by intrauterine distress-reoxygenation in rats

作　　者：卢欢[1,2] 郑晓春[2,3] 陈小琳[1] 吴希珠[1,2] 郑官林 

机构地区：[1]福建省妇幼保健院麻醉科 [2]福建医科大学省立临床学院 [3]福建省立医院麻醉科,福建福州350001

出　　处：《中国病理生理杂志》2015年第6期1120-1124,共5页Chinese Journal of Pathophysiology

基　　金：福建省自然科学基金资助项目(No.2013J01116);福建省卫生厅青年研究基金资助项目(No.2013-2-2)

摘　　要：目的:探索母体肢体缺血预处理(LIP)对宫内窘迫胎鼠复氧后海马神经元线粒体结构和功能的影响。方法:将孕20 d的40只SD大鼠随机分为假手术组(S组)、LIP对照组、胎鼠宫内窘迫组(FD组)和LIP+FD组。通过实验设计建立胎鼠宫内窘迫模型,观察各组胎鼠海马CA1区线粒体超微结构的变化;检测海马线粒体跨膜电位变化;测定海马组织活性氧簇(ROS)、三磷酸腺苷(ATP)、丙二醛(MDA)含量及锰-超氧化物歧化酶(MnSOD)活性变化。结果:(1)与S组比较,FD组和LIP+FD组电镜下观察胎鼠海马CA1区线粒体呈不同程度破坏,线粒体膜电位下降,ATP含量和Mn-SOD活性降低,ROS和MDA含量增加(P<0.05)。(2)与FD组比较,LIP+FD组胎鼠海马CA1区线粒体的超微结构保持较好的完整性,线粒体膜电位明显提高,海马ATP含量和Mn-SOD活性显著增高,ROS和MDA含量减少(P<0.05)。结论:母体肢体缺血预处理对宫内窘迫胎鼠复氧后海马神经元线粒体功能具有保护作用。AIM：To investigate the effects of maternal limb ischemic preconditioning （ LIP） on the mitochon-drial structures and functions of the hippocampal neurons induced by reoxygenation in the intrauterine distress fetal rats. METHODS：Pregnant rats （n=40） were randomly divided into 4 groups： sham （S） group, LIP group, fetal distress （ FD） group and LIP＋FD group.Intrauterine ischemia model was established through the experimental design.The ultra-structure of the mitochondria in CA1 area of the hippocampus was observed .The mitochondrial membrane potential and re-active oxygen species （ ROS） were measured .The content of ATP and MDA in the hippocampus tissue was detected.The activity of Mn-SOD was observed.RESULTS：Compared with sham group, the ultrastructure of mitochondria in CA1 area of the hippocampus was damaged in FD group and LIP＋FD group.The mitochondrial membrane potential, the content of ATP and the activity of Mn-SOD were decreased.However, the content of ROS and MDA was increased.Compared with FD group, the ultrastructure of mitochondria in CA1 area of the hippocampus was intact in LIP＋FD group.Furthermore, the reduced mitochondrial membrane potential and ATP content were inhibited.The activity of Mn-SOD was increased, but the content of ROS and MDA was decreased in LIP＋FD group.CONCLUSION：Limb ischemia preconditioning inhibits the damage the mitochondria of fetal hippocampal neurons induced by reoxygenation in the intrauterine distress fetal rats.

关 键 词：肢体缺血预处理 胎儿缺氧/复氧损伤 线粒体 

分 类 号：R364.4[医药卫生—病理学] R363.2[医药卫生—基础医学]