Yes相关蛋白在舌鳞癌组织中的表达及其意义  

Expression and significance of Yes-associated protein in tongue squamous cell carcinoma

作　　者：牛艳红[1] 周容[1] 方喜泉[1] 

机构地区：[1]惠州市中心人民医院口腔科,广东惠州516001

出　　处：《广东牙病防治》2015年第6期288-292,共5页Journal of Dental Prevention and Treatment

基　　金：惠州市科技计划项目(2014Y038)

摘　　要：目的探讨Yes相关蛋白(Yes-associated protein,YAP)在舌鳞癌组织中的表达及其在舌鳞癌发生发展的作用。方法通过实时定量逆转录多聚酶链反应(reverse transcription polymerase chain reaction,RT-PCR)、免疫组织化学及Western Blot等方法,分别检测112例舌鳞癌患者的舌鳞癌组织、癌旁组织中YAP蛋白的表达情况。结果 YAP在舌鳞癌组织中阳性表达率为92.0%(103/112),在癌旁组织阳性表达率为19.6%(22/112)。舌鳞癌组织中YAP的阳性表达率明显高于癌旁组织(χ2=44.51,P<0.01)。舌鳞癌YAP表达阳性率与患者的性别、年龄、临床分期无关,与舌鳞癌的病理分级(分化程度)有关,分化程度越低表达率越高,差异有统计学意义(χ2=11.68,P<0.01)。YAP蛋白在淋巴结转移阳性的舌鳞癌患者中表达率高(χ2=4.47,P<0.05)。结论 YAP在舌鳞癌中高表达,参与了舌鳞癌的发生发展。Objective To investigate the expression of YAP in tongue squamous cell carcinoma and its role in the oc-currence and development of squamous cell carcinoma of tongue.Methods A total of 112 patients with tongue squamous cell carcinoma were enrolled in this study.The expression of YAP protein in cancerous tissues and in adjacent tissues were detected by real-time RT-PCR, immunohistochemistry and Western Blot.Results The positive expression rate of YAP in tongue squamous cell carcinoma was 92% （ 103/112 ） .The positive expression rate of YAP was 19.6%（22/112） in adjacent tissue.The positive expression rate of YAP in tongue squamous cell carcinoma was significantly higher than that in adjacent tissue （P〈0.05）.The positive expression rate of YAP in tongue squamous cell carcinoma patients was not related to gender, age and clinical stages, but related to the pathological grade （ differentiation） of squa-mous cell carcinoma.There was a correlation between the positive expression rate of YAP and of the tongue.The lower the differentiation degree is, the higher expression rate will be, while there were significant differences between them （χ2 =11.68, P〈0.01）.The positive expression rate of YAP were higher in lymph node positive patients than those in node negative patients （χ2 =4.47, P〈0.05）.Conclusion The expression of YAP in tongue squamous cell carcinoma was high, which may be involved in the occurrence and development of squamous cell carcinoma of the tongue.

关 键 词：Yes相关蛋白 舌鳞癌 逆转录多聚酶链反应 

分 类 号：R739.86[医药卫生—肿瘤]