刚地弓形虫蛋白质组学研究进展  

Research Advances on Toxoplasma gondii Proteomics

作　　者：邱竞帆[1] 葛可 王勇[1] 

机构地区：[1]南京医科大学基础医学院病原生物学系,南京210029

出　　处：《中国寄生虫学与寄生虫病杂志》2015年第3期214-218,221,共6页Chinese Journal of Parasitology and Parasitic Diseases

基　　金：江苏省高校自然科学研究面上项目(No.13KJB310004);南京医科大学科技发展基金重点项目(No.2012NJMU015);江苏高校优势学科建设工程资助项目(PAPD)~~

摘　　要：近10年,蛋白质组学技术广泛应用于刚地弓形虫研究中,研究结果为全面深入揭示该虫体的生命活动本质奠定了基础。目前,关于刚地弓形虫的整体蛋白质组学研究仅限于速殖子和卵囊阶段的蛋白质组学;亚蛋白质组学研究包括一些重要抗原,如可溶性速殖子抗原、糖蛋白和免疫蛋白质组学研究等;差异蛋白质组学研究着眼于阐明不同虫株或不同虫种的差异蛋白。本文综述了弓形虫蛋白质组学的研究现状,详细阐述了弓形虫整体蛋白质组学、亚蛋白质组学和差异蛋白质组学的主要研究进展。此外,本文还概述了弓形虫蛋白质组学的生物信息学平台,并对弓形虫蛋白质组学的应用前景进行了展望。The proteomic techniques have been widely used in Toxoplasma gondii research since the past decade, providing proteomic data that facilitate understanding of T. gondii activities. Currently, the global proteomic studies of T. gondii are mainly confined to the tachyzoite and the oocyst stages. The subproteomic research involves some important antigens, such as the soluble tachyzoite antigen, glycoproteins, and immunoproteins, etc. The differential proteomics of T. gondii is mainly focused on identifying differentially-expressed proteins in different T. gondii strains. This review summarizes the current status of proteomic research on T. gondii, with specific focuses on global proteomic, subproteomic, and differential proteomic findings. In addition, this review gives an overview on web-based resources that provide proteomic data and support for studies on T. gondii, and finally discusses future prospects of T. gondii proteomics applications.

关 键 词：刚地弓形虫 蛋白质组学 排泄-分泌抗原 可溶性速殖子抗原 

分 类 号：R382.5[医药卫生—医学寄生虫学]