17-AAG联合紫杉醇对Eca-109食管癌细胞增殖的抑制作用  

Inhibitory effect of 17-AAG combined with paclitaxel on proliferation of esophageal squamous cell carcinoma Eca-109 cells in vitro

作　　者：陈斯泽[1] 陈雪梅[2] 李玉齐[1] 杨曙[1] 莫贤毅[1] 张帆[1] 莫凯岚[1] 丁颖[1] 

机构地区：[1]广东药学院附属第一医院肿瘤科,广东广州510080 [2]南方医科大学热带医学与公共卫生学院,广东广州510515

出　　处：《南方医科大学学报》2015年第6期844-847,共4页Journal of Southern Medical University

基　　金：国家自然科学基金(81171876)~~

摘　　要：目的研究17-AAG联合紫杉醇(PTX)对食管癌细胞Eca-109增殖和凋亡的影响。方法予以PTX和17-AAG单独或联合使用作用于Eca-109细胞株,采用MTT法检测其细胞增殖的变化,应用流式细胞仪检测细胞周期、凋亡的变化。结果与对照组相比,单独使用17-AAG、PTX均能够抑制Eca-109细胞的增殖;0.5μmol/L PTX联合0.625μmol/L 17-AAG可抑制Eca-109的生长,且联合效应明显强于各自单药组(P<0.01);流式细胞仪检测结果显示:17-AAG将Eca-109细胞阻滞于G2/M期,PTX将Eca-109细胞阻滞于S期。17-AAG与PTX联合用药使Eca-109细胞阻滞于G2/M期和S。17-AAG组、PTX组及联合组作用Eca-109细胞株24 h后其凋亡率分别为4.52%、10.91%、29.88%,显著高于对照组(1.32%);联合用药后,可形成明显凋亡峰,明显高于单药组。结论 PTX和17-AAG均可抑制食管癌细胞增殖,诱导癌细胞凋亡,两者联合可增强上述作用。Objective To investigate the effect of 17-AAG combined with paclitaxel（PTX） on the proliferation and apoptosis of esophageal squamous cell carcinoma cell line Eca-109 in vitro. Methods Eca-109 cells were treated with 17-AAG and PTX either alone or in combination. The proliferation of Eca- 109 cells was detected by MTT assay, and the cell cycle changes and cell apoptosis were determined by flow cytometry. Results Compared with the control group, both 17-AAG and PTX significantly inhibited the proliferation of Eca-109 cells. A combined treatment of the cells with 0.5 μmol/L PTX and 0.625 μmol/L 17-AAG produced an obviously stronger inhibitory effect on the cell proliferation than either of the agents used alone（P〈0.01）. Flow cytometry showed that, 17-AAG and PTX used alone caused Eca-109 cell cycle arrest in G2/M phase and S phase, respectively,and their combined use caused cell cycle arrest in both G2/M and S phases. The cell apoptosis rates of Eca-109 cells treated with17- AAG, PTX and their combination were 4.52%, 10.91%, and 29.88%, respectively, all significantly higher than that in the control group（1.32%）; the combined treatment resulted in a distinct apoptotic peak that was significantly higher than that caused by either of the agents alone. Conclusion 17-AAG and PTX can inhibit cell proliferation and promote apoptosis of Eca-109 cells, and their combination produces stronger effects in inhibiting cell proliferation and increasing cell apoptosis.

关 键 词：食管癌 17-AAG 紫杉醇 增殖 凋亡 

分 类 号：R735.1[医药卫生—肿瘤]