鼻咽癌患者外周血端粒酶hTERT mRNA的定量检测及其临床意义  

Quantitative detection of plasma level of human telomerase reverse transcriptase mRNA in patients with nasopharyngeal carcinoma

作　　者：付新洒 申聪香[1] 李冠雪[1] 张鑫雨[1] 文忠[1] 

机构地区：[1]南方医科大学珠江医院耳鼻咽喉-头颈外科,广东广州510282

出　　处：《南方医科大学学报》2015年第6期894-897,共4页Journal of Southern Medical University

基　　金：广东省自然科学基金(S2011010003947)

摘　　要：目的定量检测鼻咽癌患者外周血中端粒酶逆转录酶催化亚单位(h TERT)m RNA的表达,并探讨其在鼻咽癌中的诊断及治疗意义。方法采用实时荧光定量PCR技术(QPCR)检测33例鼻咽癌患者治疗(放疗或化疗)前后血浆中h TERT m RNA的表达,并分析其与临床病理因素的关联,以24例健康志愿者作为对照。结果结果显示,健康志愿者血浆中h TERT m RNA相对表达量为0.95±0.37,鼻咽癌患者血浆中h TERT m RNA含量达到10.75±4.29(P<0.05)。鼻咽癌患者血浆中h TERT m RNA的表达与年龄、性别无统计学差异(P>0.05),而与临床分期、T分期及N分期有显著性差异(P<0.05)。早期患者(Ⅰ、Ⅱ期),放疗后其表达量3.43±1.42较治疗前5.60±2.33明显下降;晚期患者(Ⅲ、Ⅳ期)诱导化疗及放疗后其表达量较治疗前12.68±3.08分别降为10.68±2.48、3.13±1.69(P<0.05)。结论鼻咽癌患者血浆中h TERT m RNA的表达明显升高,放化疗能够有效抑制该基因的表达,而且该基因的含量与肿瘤的临床分期、大小及淋巴结浸润范围等临床病理因素密切相关,从而提示检测鼻咽癌患者外周血中h TERT m RNA的含量,有可能为鼻咽癌患者早期诊断及疗效判断提供重要信息。Objective To quantitatively measure plasma level of human telomerase reverse transcriptase（h TERT） m RNA in patients with nasopharyngeal carcinoma（NPC） and explore its implications for NPC diagnosis and treatment. Methods With24 healthy volunteers serving as controls, the plasma level of h TERT m RNA was detected in 33 NPC patients by real-time PCR before and after treatments with chemotherapy or radiotherapy, and its association with the clinicopathological parameters of the patients were analyzed. Results The NPC patients showed a significantly higher mean plasma level of h TERT m RNA than the healthy volunteers（10.75±4.29 vs 0.95±0.37, P〈0.05）. The plasma h TERT m RNA level in the NPC patients was significantly correlated with clinical staging, tumor size, and degree of nodal metastasis（P〈0.05） but with gender or age（P〈0.05）. In patients with stage I and II NPC, the plasma h TERT m RNA level decreased significantly after radiotherapy（5.60±2.33 vs 3.43±1.42）; in patients in advanced stages（III and IV）, plasma h TERT m RNA level decreased significantly from 12.68±3.08 to 10.68±2.48（P〈0.05） after chemotherapy and to 3.13 ± 1.69（P〈0.05） after radiotherapy. Conclusion Radiotherapy and chemotherapy can effectively suppress elevated plasma h TERT m RNA levels in NPC patients. Plasma h TERT m RNA level is closely related to the clinicopathological factors and provides important information for early diagnosis and therapeutic effect evaluation of NPC.

关 键 词：鼻咽癌 血浆 实时荧光定量 HTERT MRNA 

分 类 号：R739.63[医药卫生—肿瘤]