雄激素疗法对去势雌性大鼠泪膜稳定性的改善作用  

Ameliorative effect of androgen therapy tear on film stability in castrate female rats

作　　者：高阳 周瑾 孙晓芳[3] 

机构地区：[1]中山市南朗医院眼科,528451 [2]广州市妇女儿童医疗中心眼科,510623 [3]中山市博爱医院眼科,528403

出　　处：《中华实验眼科杂志》2015年第7期595-599,共5页Chinese Journal Of Experimental Ophthalmology

基　　金：国家自然科学基金项目（81200666）;中山市科技局项目（20122A088）

摘　　要：背景 绝经期女性干眼的发病率明显高于男性,提示性激素在干眼的发病过程中可能发挥一定的作用,绝经期女性应用雌激素时于眼症状加重也提示雄激素可能参与干眼病程.雄激素的应用是否可以减轻干眼症状正在受到关注. 目的 探讨雄激素对去卵巢雌鼠泪液及泪膜稳定性的影响,探讨雄激素维持泪膜稳定性的可能作用机制. 方法 清洁级3月龄雌性性成熟Wistar大鼠48只按照随机数字表法分为正常对照组、假手术组、去势模型组和丙酸睾丸酮组,去势模型组和丙酸睾丸酮组大鼠行双侧卵巢摘除术以制备去势模型,造模后5同始丙酸睾丸酮组大鼠按3.75 mg/kg剂量肌内注射丙酸睾丸酮,每3天1次,持续6周,而假手术组大鼠仅切除腹内脂肪组织而不摘除卵巢.于丙酸睾丸酮组大鼠给药后6周时测定各组大鼠血清雄激素质量浓度,检测并比较各组大鼠Schirmer试验Ⅰ（SⅠt）、泪膜破裂时间（BUT）结果.于丙酸睾丸酮组大鼠给药前及给药后6周时各组过量麻醉法任意处死10只大鼠,制备结膜和角膜组织切片,行角膜结膜免疫荧光染色,检测各组大鼠结膜表面MUC5AC的阳性表达;采用扫描电子显微镜检测角膜细胞表面的超微结构改变.实验动物的使用和喂养遵循南方医科大学实验动物管理细则,并通过南方医科大学实验动物伦理委员会审核批准.结果 去势模型组大鼠血清睾丸酮质量浓度为（1.83±0.12） ng/ml,SⅠt值为（3.63±0.26） mm/5 min,BUT值为（3.73±0.38）s,分别低于正常对照组的（2.56±0.14） ng/ml、（7.47±0.66） mm/5 min和（9.57±0.76）s,差异均有统计学意义（均P=0.000）;而丙酸睾丸酮组大鼠血清睾丸酮质量浓度为（3.38±0.24） ng/ml,SⅠt值为（6.37±0.45） mm/5 min,BUT值为（7.54±0.55）s,均明显高于去势模型组,差异均有统计学意义（均P=0.000）.去势模型组大鼠结膜组织中MUC5AC阳性染色强度明显较正常Background Prevalence of dry eye is significantly inceasing in postmenopausal women than that in men,suggesting that sex hormone plays a role in the pathogenesis of dry eye.In addition,dry eye might become worse following estrogen therapy in postmenopausal women.However,whether application of androgen can ameliorate dry eye is being concerned.Objective This study was to investigate the effect of androgen on tear film of ovariectomized female rats.Methods Forty-eight 3-month-old sexually mature female Wistar rats were randomized into the normal control group,sham group,ovariectomy （OVX） model group and testosterone-injected group.OVX models were established by bilateral ovaries enucleation in the rats of the model group and testosterone-injected group,and then androgen （3.75 mg/kg） was intramuscularly injected since 5 months after OVX at 3-day interval for 6 weeks.Only intraperitoneal fat was cut off in the sham group.In 6 weeks after injection of androgen,serum androgen concentration detected and Schirmer Ⅰ test （S Ⅰ t）,tear film break-up time （BUT） were performed.The rats were sacrificed to prepare the corneal and conjunctival samples.The expression of MUC5AC in conjunctival tissue was examined by immunofluorescence staining,and the microstructure of corneal cellular surface was observed under the scanning electron microscope before and 6 weeks after application of androgen.Animals in this study were treated in accordance with Animal Experimentation Ethic Committee Guidelines of Southern Medical University and the study protocol was approved by Ethic Committee of this University.Results The mean serum testosterone concentration was （1.83 ±0.12） ng/ml,and S Ⅰ t or BUT was （3.63-±0.26）mm/5 minutes or （3.73-0.38） seconds,respectively,in the OVX model group,which was significantly declined in comparison with （2.56 ±0.14） ng/ml,（7.47±0.66） mm/5 minutes or （9.57 ±0.76） seconds in the normal control group （all at P =0.000）.However,the serum testosterone concentrati

关 键 词：干眼/治疗 泪液/分泌 卵巢切除术 雄激素/药物疗法 动物模型 WISTAR大鼠 雌性 

分 类 号：R777.34[医药卫生—眼科]