IGF-1和IGF-1R在无功能性垂体腺瘤中的表达及意义  

Expression of insulin-like growth factor 1 and its receptor in human non-functioning pituitary adenomas: correlation with invasiveness and recurrence

作　　者：王志明[1] 蒲九君 孙彦龙[1] 周辉[1] 靳凯[1] 阮伦亮 钟艾凌 杨刚[1] 

机构地区：[1]重庆医科大学附属第一医院神经外科,重庆400016

出　　处：《第三军医大学学报》2015年第13期1367-1372,共6页Journal of Third Military Medical University

基　　金：国家临床重点专科建设项目(财社[2011]170号);重庆市科委自然科学基金(CSTC2013jcyj A10079)~~

摘　　要：目的探讨胰岛素样生长因子1(insulin-like growth factor 1,IGF-1)和胰岛素样生长因子1型受体(insulin-like growth factor 1 receptor,IGF-1R)与无功能性垂体腺瘤侵袭性和复发性的关系。方法60例无功能性垂体腺瘤分为侵袭性复发性组(16例)、侵袭性非复发性组(24例)和非侵袭性非复发性组(20例)。采用qRT-PCR检测IGF-1和IGF-1R的mRNA表达水平,采用免疫组织化学法和Western blot检测IGF-1、IGF-1R和其下游信号分子血管内皮生长因子(vascular endothelial growth factor,VEGF)的蛋白表达水平。结果 IGF-1和IGF-1R的mRNA表达水平及IGF-1、IGF-1R和VEGF的蛋白表达水平,侵袭性复发性组高于侵袭性非复发性组(P<0.05),侵袭性非复发性组高于非侵袭性非复发性组(P<0.05)。相关性分析结果显示,在侵袭性复发性、侵袭性非复发性无功能性垂体腺瘤组织中IGF-1和IGF-1R蛋白表达量呈正相关(r=0.85,r=0.80,P<0.05),IGF-1R和VEGF蛋白表达量呈正相关(r=0.74,r=0.70,P<0.05);在非侵袭性非复发性无功能性垂体腺瘤组织中IGF-1和IGF-1R蛋白表达量呈正相关(r=0.73,P<0.05),IGF-1R和VEGF蛋白表达量无明显相关性(P>0.05)。结论IGF-1、IGF-1R和VEGF在侵袭性和复发性无功能性垂体腺瘤中表达上调,三者协调作用,与无功能性垂体腺瘤的侵袭性和复发性恶性行为的发生、发展可能相关。Objective To investigate the expression levels of insulin-like growth factor 1（ IGF-1） and its receptor（ IGF-1R） in human non-functioning pituitary adenomas and their relationships with tumor invasion and recurrence. Methods Sixty specimens of non-functioning pituitary adenomas collected in surgery in our department from August 2012 to May 2014 were classified into invasive recurrent group（ n = 16）,invasive nonrecurrent group（ n = 24） and non-invasive non-recurrent invasive group（ n = 20） according to the clinical data of the corresponding patients. Quantitative real-time polymerase chain reaction（ qRT-PCR） was used to detect the mRNA expression levels of IGF-1 and IGF-1R. Western blot assay and immunohistochemical assay were employed to measure the protein levels of IGF-1,IGF-1R and its downstream molecule of associated signaling pathway,VEGF. Results The mRNA expression levels of IGF-1 and IGF-1R and the protein expression levels of IGF-1,IGF-1R and VEGF were significantly higher in invasive recurrent groups than in invasive nonrecurrent group（ P〈0. 05）,and in the invasive non-recurrent group than non-invasive non-recurrent group（ P〈0. 05）. The correlation analysis revealed that there was a positive correlation between the protein levels of IGF-1and IGF-1R（ r = 0. 85,r = 0. 80,P〈0. 05）,and between those of IGF-1R and VEGF（ r = 0. 74,r =0. 70,P〈0. 05） in invasive recurrent group and invasive non-recurrent group respectively. Positive correlation was also found between the protein levels of IGF-1 with IGF-1R in non-invasive non-recurrent group（ r = 0. 73,P〈0. 05）,but no obvious relation was found in the levels of IGF-1R with VEGF（ P〈0. 05）. Conclusion The expression of IGF-1,IGF-1R and VEGF is enhanced in the invasiveness and recurrence of non-functioning pituitary adenomas. The 3 molecules may play synergetic roles and are involved in the occurrence and development of invasive growth and recurrence of the tumor.

关 键 词：IGF-1 IGF-1R 无功能性垂体腺瘤 侵袭性 复发性 

分 类 号：R394.3[医药卫生—医学遗传学] R730.23[医药卫生—基础医学]