高温对实验小鼠精液质量影响的研究  

Effect of high temperature state on semen quality in laboratory mice

作　　者：陈杰翔[1] 李利[2] 

机构地区：[1]泸州医学院附属医院泌尿外科,四川泸州646000 [2]泸州医学院附属医院护理部,四川泸州646000

出　　处：《牡丹江医学院学报》2015年第3期25-28,共4页Journal of Mudanjiang Medical University

摘　　要：目的本研究以成年雄性昆明小鼠构建不同高温状态下的实验动物模型,测定精子DNA及精子顶体完整率的变化情况,探讨在不同高温状态下小鼠精液质量的变化情况,为研究高温状态影响人类生育能力的机理提供动物实验依据。方法将125只健康雄性成年昆明小鼠随机分为五组,每组25只,其中对照组予以22°C恒温饲养,高温组下设31°C、34°C、37°C、40°C四个组,分别于实验开始的第1、3、5、7、9天随机抽取5只测定每组实验小鼠精子DNA及精子顶体完整率的变化情况。结果 1、与对照组相比较,各高温组实验小鼠的精液质量均明显异常,且温度越高,精液质量下降越快,差异具有统计学意义(P<0.01);2、随着高温持续时间的延长,各高温组实验小鼠的精液质量呈现不同程度的异常,差异具有统计学意义(P<0.01)。结论高温可能通过影响雄性小鼠精子DNA损伤及附属性腺的功能,导致精子DNA及精子顶体完整率出现不同程度的异常,从而影响其生育能力。Objective To use adult male mice to establish different experimental animal models under high temperature , and to explore the effect of different temperature on semen quality in mice and to provide human fertility based on animal experiments .Mteh-ods The 125 healthy adult male mice were randomly divided into five groups （ each group 25） .The control group was treated with 22℃thermo-stat feeding , the high -temperature groups were divided into 31℃, 34℃, 37℃, 40℃four groups , respectively .At the days 1, 3, 5, 7 and 9, five mice per group were randomly selected to measure the changes in sperm DNA integrityand and acrosome integrity rate.Results Compared with the control group , the semen quality of mice in high -temperature experiments were signifi-cantly abnormal ,and the higher the temperature ,the faster the decline in semen quality ,the difference was statistically significant （ P〈0.01）;2.With the high temperature duration ,the extended semen quality of mice in high -temperature experiments showed varying degrees of abnormality , the difference was statistically significant （ P〈0.01 ） .Conclusion High temperature may influence male sperm DNA damage and its subsidiaries gonadal function , which lead to sperm DNA integrity and sperm acrosome integrity rate varying degrees of abnormality ,thus affecting their fertility.

关 键 词：高温 精液分析 精子DNA 精子顶体完整率 

分 类 号：R73[医药卫生—肿瘤]