β-拉帕醌对胃癌细胞生长与侵袭的抑制作用及机制  

Inhibitory effects of β-lapachone on the growth and invasion in gastric cancer cells

作　　者：秦云植[1,2] 杨洋[1] 朴俊杰[1] 李珍玲[1] 崔雪莲[1] 林贞花[1] 

机构地区：[1]延边大学医学院病理学教研室,延吉133000 [2]延边大学附属医院麻醉科,延吉133002

出　　处：《临床与实验病理学杂志》2015年第6期601-606,共6页Chinese Journal of Clinical and Experimental Pathology

基　　金：国家科技部"973计划"前期研究专项(2014CB560708);国家自然科学基金(61371067)

摘　　要：目的探讨β-拉帕醌体外抑制胃癌细胞增殖和迁移及诱导凋亡的作用及机制。方法应用噻唑蓝(MTT)及平板克隆实验检测β-拉帕醌对SGC-7901与AGS胃癌细胞增殖的影响,划痕实验检测β-拉帕醌抑制胃癌细胞的迁移能力,流式细胞术检测β-拉帕醌诱导胃癌细胞凋亡的作用。应用Western blot法检测β-拉帕醌处理胃癌细胞前后其增殖、迁移、上皮-间质转化(epithelial-mesenchymal transition,EMT)及凋亡分子标志物的变化。结果β-拉帕醌可显著抑制SGC-7901和AGS胃癌细胞的增殖能力,并下调增殖与周期相关Skp2和DEK蛋白的表达(P均<0.05);经β-拉帕醌处理后,胃癌细胞的迁移能力明显下降,且显著下调MMP-2/9和Ezrin蛋白以及EMT间质标志物的表达,上调EMT上皮标志物表达水平;另外,β-拉帕醌增加胃癌细胞的凋亡,下调BCL-2/Bax比值以及上调活化型Caspase-3/8/9的表达。结论β-拉帕醌对胃癌细胞有明显的抑制增殖及诱导凋亡的作用,并可通过MMPs和EMT途径抑制胃癌细胞的迁移能力。Purpose To investigate the effects of β-lapachoneon inhibition of proliferation and migration and induction of apoptosis in gastric cancer cells in vitro. Methods The cell viability was detected using MTT and colony formation assay,the migration ability was determined using scratch assay method,and the apoptosis was examined using flow cytometry. Meanwhile,the expression of biomarkers of proliferation,EMT markers andapoptosiswere detected using Western blot analysis. Results β-lapachonecould significantly inhibit the proliferation of SGC-790l and AGS gastric cancer cells（ P〈0. 05）,and down-regulate the expression levels of Skp2 and DEK pro-teins. β-lapachoneould also inhibited the invasion and motility of gastric cancer cells via down-regulating the expression levels of MMP-2/9 and Ezrin proteins and up-regulating the epithelial markers. In addition,β-lapachoneenhanced the apoptosis of gastric canc-er cells,down-regulation of BCL-2/Bax ratio and up-regulation of activated Caspase-3/8/9. Conclusions β-lapachonecan effectively inhibit the proliferation and induce the apoptosis of gastric cancer cells,and inhibit the migration of gastric cancer cells via MMPs and EMT pathways.

关 键 词：胃肿瘤 β-拉帕醌 增殖 上皮-间质转化 细胞凋亡 

分 类 号：R737.33[医药卫生—肿瘤]