Synchrotron radiation X-ray fluorescence analysis of Fe, Zn and Cu in mice brain associated with Parkinson's disease  

作　　者：田甜 张继超 雷豪志 诸颖 施继晔 胡钧 黄庆 樊春海 孙艳红 

机构地区：[1]Key Laboratory of Interfacial Physics and Technology,Chinese Academy of Sciences [2]Shanghai Institute of Applied Physics, Chinese Academy of Sciences [3]University of Chinese Academy of Sciences [4]UCB Pharm

出　　处：《Nuclear Science and Techniques》2015年第3期100-105,共6页核技术（英文）

基　　金：Supported by the Ministry of Science and Technology of China(Nos.2012CB825805 and 2012CB932600);National Natural Science Foundation of China(Nos.11179004,21390414,U1232113,U1232114,U1332119 and U1432116);Youth Innovation Promotion Association of CAS

摘　　要：The contents and distributions of metal elements in the brain are closely related to neurodegenerative diseases.In this study, we examined Fe, Cu and Zn contents in the brain section associated with Parkinson‘s disease(PD)using synchrotron radiation X-ray fluorescence(SRXRF). PD mouse model induced by 1-methyl-4-phenyl-1,2,3,6-terahydropyridine(MPTP) was used for the elemental analysis(e.g., Fe, Cu and Zn) in the substantia nigra pars compacta(SNpc) region of mice brain tissue samples. We found that mice in the MPTP group had higher contents of Fe, Cu and Zn in the SNpc than the control group. After treating the PD mice with rapamycin, the contents of Fe, Cu and Zn were reduced, the dopamine neurons and motor function were rescued correspondingly. The results prompted that the SRXRF provided an ideal method for tracing and analyzing the metal elements in the brain section to assess the pathological changes of PD model and the therapeutic effect of drugs.The contents and distributions of metal elements in the brain are closely related to neurodegenerative diseases.In this study, we examined Fe, Cu and Zn contents in the brain section associated with Parkinson‘s disease（PD）using synchrotron radiation X-ray fluorescence（SRXRF）. PD mouse model induced by 1-methyl-4-phenyl-1,2,3,6-terahydropyridine（MPTP） was used for the elemental analysis（e.g., Fe, Cu and Zn） in the substantia nigra pars compacta（SNpc） region of mice brain tissue samples. We found that mice in the MPTP group had higher contents of Fe, Cu and Zn in the SNpc than the control group. After treating the PD mice with rapamycin, the contents of Fe, Cu and Zn were reduced, the dopamine neurons and motor function were rescued correspondingly. The results prompted that the SRXRF provided an ideal method for tracing and analyzing the metal elements in the brain section to assess the pathological changes of PD model and the therapeutic effect of drugs.

关 键 词：同步辐射X射线荧光分析 帕金森病 锌含量 鼠脑 低铁 神经退行性疾病 小鼠模型 Fe含量 

分 类 号：R742.5[医药卫生—神经病学与精神病学]