OPG及RANKL在小儿单纯性骨囊肿与动脉瘤样骨囊肿中的表达研究  

The expression of osteoprotegerin(OPG) and receptor activator nuclear factorK-B ligand(RANKL) in pediatric bone tumor-like lesions(simple bone cysts and aneurysmal bone cysts)

作　　者：宋得夫[1] 毕波[2] 邵景范[2] 杨小进[2] 王小林[2] 

机构地区：[1]西安市儿童医院骨科,陕西省西安市710003 [2]华中科技大学同济医学院附属同济医院小儿外科,湖北省武汉市430030

出　　处：《临床小儿外科杂志》2015年第3期194-197,共4页Journal of Clinical Pediatric Surgery

基　　金：国家临床重点专科建设项目基金资助(国卫医函[2013]544)

摘　　要：目的：检测小儿单纯性骨囊肿和动脉瘤样骨囊肿标本中骨保护素（OPG）、核因子-kB 受体活化因子配体（RANKL）的表达水平，并与正常骨组织作对照，分析两种蛋白在这两种瘤样病变中的表达是否存在差异及意义。方法共收集小儿单纯性骨囊肿标本31例，动脉瘤样骨囊肿标本23例，正常骨组织标本（对照组）14例。采用免疫组化 SABC 方法检测单纯性骨囊肿、动脉瘤样骨囊肿和对照组中 OPG、RANKL 的表达，并分析其差异性。应用 Imagepro-Plus 6．0图像处理软件及数据处理软件SPSS17．0对图像及所得数据进行分析。结果在单纯性骨囊肿及动脉瘤样骨囊肿中，OPG 的表达量均低于正常骨组织，RANKL 的表达量均高于正常骨组织，其中动脉瘤样骨囊肿 RANKL 的表达量较单纯性骨囊肿更高，差异有统计学意义（P ＜0．05）；单纯性骨囊肿的 OPG 稍高于动脉瘤样骨囊肿，差异无统计学意义（P ＞0．05）。RANKL／OPG 比值动脉瘤样骨囊肿＞单纯性骨囊肿＞正常组织，差异有统计学意义（P ＜0．05）。结论RANKL／OPG 失衡可能是小儿单纯性骨囊肿和动脉瘤样骨囊肿出现溶骨性破坏的原因之一，动脉瘤样骨囊肿术后相对更高的复发率可能与 RANKL 的相对高表达有关。Objetive To explore the expression of osteoprotegerin （OPG）and receptor activator nuclear factor κ-B ligand （RANKL）in pediatric bone tumor-like lesions （bone cysts,aneurysmal bone cysts）. Methods 31 specimens of simple bone cysts,23 of aneurysmal bone cysts were collected during surgery and were taken as experimental group.Normal bone tissues from 14 patients were collected as normal controls.The expressions of OPG and RANKL were detected by immunohistochemical staining and quantified by computer image analysis software.Results Compared with normal controls,the expression of RANKL was significantly higher in simple bone cysts and aneurysmal bone cysts,whereas the expression of OPG was significantly lower （P 〈0.05）.The expression of RANKL of aneurysmal bone cysts was significantly higher than that of simple bone cysts （P 〈0.05）.The expression of OPG of simple bone cysts was slightly higher than that of aneurysmal bone cysts,but the difference was not statistically significant （P 〉0.05）.The aneurysmal bone cysts had the highest ratio of RANKL to OPG,followed by simple bone cysts,then the normal controls （P 〈0.05）. Con-clusions Abnormal expression of RANKL and OPG is associated with pediatric bone tumor-like lesions like simple bone cysts and aneurysmal bone cysts.

关 键 词：骨囊肿 动脉瘤样 骨和骨组织 骨保护素 儿童 

分 类 号：R738.1[医药卫生—肿瘤]