FTY720抑制S1P诱导的角膜新生血管的研究  

Effect of FTY720 inhibiting corneal neovascularition induced by sphingosine 1-phosphate

作　　者：钟帆[1] 丁小珍[1] 杨为中[1] 高宗银[1] 陆晓和[2] 

机构地区：[1]广州医科大学附属广州市第一人民医院眼科,中国广东省广州市510180 [2]南方医科大学附属珠江医院眼科,中国广东省广州市510180

出　　处：《国际眼科杂志》2015年第7期1134-1138,共5页International Eye Science

基　　金：广东省医学科学技术研究基金(No.B2011269);广州市医药卫生科技项目(No.201102A213104)~~

摘　　要：目的:探讨芬戈莫德(fingolimod,FTY720)对大鼠角膜新生血管(CNV)的抑制作用。方法:分别采用MTT法和划痕法观察不同浓度FTY720对人脐静脉内皮细胞(HUVECs)的增生和S1P诱导下的细胞迁移的影响。应用大鼠角膜微囊袋模型,检测FTY720对S1P诱导的CNV的作用。将30只SD大鼠按随机数字表法分成A、B和C组,每组10只,在各组角膜基质层内植入S1P的同时依次植入0,5,20μg FTY720缓释颗粒。术后对CNV生长情况观察,并在12d行组织病理学检查。实验结果采用单因素方差分析。结果:10,102,103,104nmol/L FTY720与HUVECs共孵育72h可抑制细胞增生(P<0.01),10,100nmol/L FTY720作用24h后,可抑制由S1P诱导的细胞迁移,随FTY720浓度增加其抑制细胞增生和迁移的作用均增强,A、B、C组大鼠角膜微囊袋缓释微粒体植入后12d,CNV面积分别为10.05±1.19,6.59±0.95,2.70±0.68mm2(F=145.155,P<0.01),A与B组、B与C组间均有统计学差异(P<0.01)。结论:FTY720能抑制S1P诱导的角膜新生血管生成。AIM ： To explore the inhibiting effect of FTY720 on corneal neovascularization（ CNV） of rat.METHODS： MTT assay and cells scratch were adopted to observe hyperplasia of human umbilical vein endothelial cells（ HUVECs） and cell migration induced by sphingosine-1-phosphate（ S1P） after using FTY720 of different concentration. The effect of FTY720 on CNV induced by S1 P in a rat corneal micropocket model was detected. 30 SD rats were randomly divided into group A,group B and group C with 10 rats per group. S1 P and0μ g,5μ g,and 20μ g FTY720 controlled-released particles were implanted into the corneal strom a. The grow th of CNV and having pathological exam ination on 12d after the operation was observed. Findings was analyzed by one-w ay ANOVA.2RESULTS： 10,10,103,and 104 nm ol / L FTY720 and HUVECs co- incubate 72 h could inhibit cell proliferation（ P 0. 01）,24 h after the function of 10,100 nmol /L FTY720,it could inhibit S1P- induced cell migration and the ability of restricting cell proliferation and cell migration was enhanced with increasing concentration of FTY720. On 12 d,after rat cornealicropocket controlled-release particles was im planted into groups A,B,C,the CNV area were respectively 10. 05±1. 19,6. 59±0. 95,2. 70±0. 68 m m^2（ F = 145. 155,P〈0.01）,group A and group B was statistically different and this was the same case betw een group B and group C（ P〈0. 01）.CONCLUSION： FTY720 can inhibit S1P-induced corneal neovascularization.

关 键 词：角膜新生血管化 芬戈莫德 1-磷酸鞘氨醇 人脐静脉内皮细胞 

分 类 号：R772.2[医药卫生—眼科]