Nrf2和Keap1在喉癌组织中的表达及意义  

The expression of Nrf2 and Keap1 in laryngeal carcinoma and its clinical indication

作　　者：袁源[1] 单春光[1] 徐鸥[1] 王静妙[1] 张海忠[1] 

机构地区：[1]河北医科大学第二医院耳鼻咽喉科,石家庄050000

出　　处：《临床耳鼻咽喉头颈外科杂志》2015年第13期1148-1151,共4页Journal of Clinical Otorhinolaryngology Head And Neck Surgery

摘　　要：目的：探讨Keap1-Nrf2-ARE信号通路在喉鳞状细胞癌发生、发展中的作用。方法：35例喉癌组织及癌旁组织取自2012-02-2013-09期间河北医科大学第二医院住院手术的喉癌患者,采用免疫组织化学方法检测喉鳞状细胞癌组织及癌旁正常组织中Nrf2和Keap1的表达情况。所有实验数据用SPSS 13.0统计软件分析。结果：Nrf2和Keap1在癌组织中的阳性表达率分别为77.14%和71.43%,在癌旁组织中的阳性表达率为8.57%和31.43%,2者相比差异有统计学意义（P〈0.01）。Nrf2和Keap1在Ⅰ-Ⅱ期喉癌组织中的阳性表达率为65.00%和55.00%,在Ⅲ-Ⅳ期喉癌组织中均为93.33%,组间比较差异有统计学意义（P〈0.05）。Nrf2和Keap1在有淋巴结转移的喉癌组织中阳性表达率均为100.00%,与无淋巴结转移的喉癌组织中阳性表达率68.00%和60.00%相比,均差异有统计学意义（P〈0.05）。Nrf2和Keap1的阳性表达与喉癌的病理分化程度、患者吸烟量以及年龄未见相关性（P〉0.05）。结论：Keap1-Nrf2-ARE信号通路所介导的氧化应激反应与喉癌的发展关系密切。Objective：To investigate the role of Keap1-Nrf2-ARE pathway in laryngeal carcinoma occurrence and development.Method：Thirty-five laryngeal carcinoma samples and para-carcinoma tissues were taken from the patients who accepted operation treatment in our hospital from Feb 2012 to Sep 2013.The expression levels of Nrf2 and Keap1were detected in 35 cases of laryngeal carcinoma with SP immunohistochemical methods.The data were analyzed by the SPSS 13.0statistical software.Result：The expression of Nrf2 in the 35 cases of laryngeal carcinoma was 77.14%,which in the para-carcinoma tissues was 8.57%,the difference between these two groups was statistically significant（P〈0.01）.The expression of Keap1 in the 35 cases of laryngeal carcinoma was 71.43%,which in the para-carcinoma tissues was 31.43%,the difference between these two groups was statistically significant（P〈0.01）.The expression of Nrf2inⅠ-Ⅱ stage was 65.00% and in Ⅲ-Ⅳ stage was 93.33%,the difference was significant（P〈0.05）.The expression of Keap1inⅠ-Ⅱ stage was 55.00% and in Ⅲ-Ⅳ stage was93.33%,the difference was significant（P〈0.05）.Of the 35 cases,the positive expression rate of Nrf2 and Keap1in laryngeal carcinoma with lymphnode metastasis were both 100.00%,compared with those without lymph nodes metastasis 68.00% and 60.00%,the difference was significant（P〈0.05）.The expression of Nrf2 and Keap1had no relationship with tumor differentiation,smoking and patient age（P〉0.05）.Conclusion：Keap1-Nrf2-ARE pathway may play an important role in progression of laryngeal squamous cell carcinoma.

关 键 词：喉癌 免疫组织化学 NRF2 KEAP1 

分 类 号：R739.6[医药卫生—肿瘤]