喉癌切缘组织中Survivin和基质金属蛋白酶-2表达与喉癌局部复发的关系  

Relationship of local recurrence with the expression of Survivin and MMP-2 in laryngeal carcinoma and its surgical margins

作　　者：周彬[1] 侯学东[1] 时胜武[1] 杨帆[1] 

机构地区：[1]沈阳医学院附属中心医院耳鼻咽喉科,沈阳110024

出　　处：《临床耳鼻咽喉头颈外科杂志》2015年第13期1160-1163,共4页Journal of Clinical Otorhinolaryngology Head And Neck Surgery

基　　金：沈阳医学院科学研究基金(No:20111020)

摘　　要：目的:探讨喉癌切缘组织中Survivin和基质金属蛋白酶-2(MMP-2)的表达与喉癌局部复发的关系。方法:选择48例原发灶为喉鳞状细胞癌、手术切缘组织病理学肿瘤阴性的患者,对喉癌组织及切缘组织应用免疫组织化学法分别进行Survivin和MMP-2检测。结果:148例喉癌患者原发灶组织中Survivin和MMP-2的阳性率分别为70.83%(34/48)、66.67%(32/48);手术切缘组织中Survivin和MMP-2的阳性率分别为47.92%(23/48)、37.50%(18/48),2组之间差异有统计学意义(P<0.05)。2喉癌原发灶组织中Survivin(+)34例及MMP-2(+)32例的复发率分别为26.47%(9/34)和25.00%(8/32),高于阴性组的7.14%(1/14)和12.50%(2/16),差异无统计学意义。3喉癌切缘组织中Survivin(+)23例及MMP-2(+)18例的复发率分别为34.78%(8/23)和38.89%(7/18),高于阴性组的8.00%(2/25)和10.00%(3/30),差异有统计学意义(P<0.05)。相关分析提示,喉癌切缘组织中Survivin阳性和MMP-2阳性与喉鳞状细胞癌的局部复发密切相关。结论:喉癌手术切缘组织中Survivin阳性、MMP-2阳性者局部复发率高,二者在手术切缘的表达可作为预测喉癌局部复发的生物学标志。Objective：To explore the relationship of the local recurrence with the expression of protein Survivin and MMP-2in the primary lesions and the surgical margins of laryngeal carcinoma.Method：The primary lesions and the surgical margins of laryngeal carcinoma of 48 patients were made into serial sections.Immunochemical methods was used to detect the expression of protein Survivin and MMP-2in the primary lesion and the surgical margins of laryngeal carcinoma of 48 patients.Result：The positive expression for Survivin and MMP-2in the primary lesion was 70.83%（34/48）and 66.67%（32/48）respectively,and the positive expression of Survivin and MMP-2in the surgical margins of laryngeal carcinoma was 47.92%（23/48）and 37.50%（18/48）,which in the primary lesion was significantly higher than those of the surgical margins of laryngeal carcinoma（P〈0.05）.The recurrence rates of primary lesion positive for Survivin（34cases）and MMP-2（32cases）were 26.47%（9/34）and25.00%（8/32）,which were higher than negative for them 7.14%（1/14）and 12.50%（2/16）（P〉0.05）.The recurrence rates of those with Survivin（23cases）and MMP-2（18cases）positive surgical margins were 34.78%（8/23）and 38.89%（7/18）respectively,which were significantly higher than those with negative ones 8.00%（2/25）and 10.00%（3/30）（P〈0.05）.Logistic analysis showed that the expression of Survivin and MMP-2protein in the surgical margins of laryngeal carcinoma was positively associated with the recurrence rates.Conclusion：Laryngeal carcinoma patients with Survivin-positive or MMP-2-positive margin would have a higher recurrence rate.Survivin and MMP-2protein can be used as biomarkers for local recurrence of laryngeal carcinoma after operation.

关 键 词：生存素 基质金属蛋白酶-2 喉肿瘤 

分 类 号：R739.65[医药卫生—肿瘤]