胰蛋白酶对皮肤鳞状细胞癌A431细胞生长的影响  

Effect of trypsin on the growth of a skin squamous cell carcinoma cell line A431

作　　者：张捷[1] 曹一鑫[1] 秦婧[2] 王建力[1] 陈莉[2] 

机构地区：[1]南通大学附属医院皮肤性病科,226001 [2]南通大学附属医院病理科,226001

出　　处：《中华皮肤科杂志》2015年第7期493-495,共3页Chinese Journal of Dermatology

基　　金：南通市社会事业科技创新与示范项目（HS2014004）

摘　　要：目的探讨胰蛋白酶对皮肤鳞状细胞癌A431细胞增殖、迁移和黏附能力等生物学行为的影响。方法用不同浓度的胰蛋白酶处理A431细胞，通过CCK8法检测细胞活性，筛选胰蛋白酶作用的最佳浓度；流式细胞仪检测癌细胞增殖周期细胞百分率和增殖率；划痕和Transwell小室实验分别检测癌细胞在二维和三维空间的迁移能力；纤连蛋白粘附试验检测癌细胞的黏附潜能。结果用不同浓度胰蛋白酶处理A431细胞，通过检测细胞生长活性发现，随着胰蛋白酶浓度升高，A431细胞增殖活性增加；100nmol／L胰蛋白酶处理A431细胞后，与对照组相比，G1期细胞比例减少，S期细胞比例增高，细胞增殖指数、细胞迁移能力和黏附潜能增加，差异均有统计学意义（均P〈0．05）。结论胰蛋白酶能促进A431细胞增殖、迁移和黏附。Objective To evaluate the effect of trypsin on the proliferation, migration and adhesion of a skin squamous cell carcinoma cell line A431. Methods Cultured A431 cells were divided into several experimental groups treated with trypsin at concentrations of 0.1, 1, 10 and 100 nmol/L for 24, 48 and 72 hours respectively, and a control group treated with DMEM complete medium only. Cell counting kit-8 （CCK8） assay was conducted to evaluate cellularproliferative activity to select the optimal concentration of trypsin. Then, some A431 cells treated with trypsin at the selected concentration for 24, 48 and 72 hours respectively （or 48 hours only） served as the experimental groups （or group）, and other A431 cells treated with DMEM complete medium served as the control group. Flow cytometry was performed to assess cell cycle distribution and proliferation index, fibronectin-based adhesion assay to estimate cell adhesive capacity, and wound healing assay and Transwell assay were conducted to evaluate the migratory capacity of cells in two- and three-dimensional space. Statistical analysis was earned out by using analysis of variance, paired samples t test and chi-square test. Results The proliferative activity of A431 cells increased along with the increase of trypsin concentrations, with the strongest increasing effect observed at 100 nmol/L. After treatment with 100 nmol/L trypsin, the experimental group showed a decrease in the percentage of Gl-phase cells, but an increase in the percentage of S-phase cells, proliferation index, migratory and adhesive capacity compared with the control group （all P 〈 0.05）. Conclusion Trypsin can promote the proliferation, migration and adhesion of A431 cells.

关 键 词：癌 鳞状细胞 胰蛋白酶 细胞生长过程 细胞周期 细胞运动 细胞系 肿瘤 

分 类 号：R739.5[医药卫生—肿瘤]