胃癌患者外周血循环肿瘤细胞检测及其临床意义  

Detection of Circulating Tumor Cells in Peripheral Blood of Patients with Gastric Carcinoma and Its Clinical Significance

作　　者：韩鸿彬[1] 李朝辉[1] 刘帅锋[1] 董帅军[1] 

机构地区：[1]郑州大学附属洛阳中心医院胃肠胰腺外科,河南洛阳471009

出　　处：《中国普外基础与临床杂志》2015年第7期840-843,共4页Chinese Journal of Bases and Clinics In General Surgery

摘　　要：目的观察胃癌患者外周血循环肿瘤细胞(CTCs)的检出情况,探讨其与胃癌临床病理特征的关系。方法选取2011年9月至2013年9月期间在笔者所在医院经病理学检查证实的60例胃癌患者为研究对象,并与同期40例胃良性疾病对照;采集其静脉血,经Cell Tracks Auto Prep系统检测其CTCs阳性率,并分析CTCs与胃癌临床病理特征的关系。结果胃癌组CTCs检出率为70.0%(42/60),对照组CTCs检出率为7.5%(3/40),胃癌患者外周血组CTCs检出率显著高于胃良性疾病者(P<0.05)。胃癌患者外周血CTCs检出率与患者性别、年龄、N分期、远处转移、肿瘤大小及脉管侵犯均无关(P>0.05),而与肿瘤TNM分期及分化程度有关(P<0.05)。外周血CTCs检测阴性的胃癌患者,其术后12个月及18个月的累积生存率均高于CTCs检测阳性者(P<0.05)。结论 CTCs检测方便,胃癌患者外周血中CTCs检出率较高,其外周血CTCs的检出情况可以反应胃癌的进展程度,可作为其预后判断的指标。Objective To observe the detection of circulating tumor cells （CTCs） in peripheral blood of patients with gastric cancer, and to investigate the relationship between the CTCs and clinicalpathological features of gastric cancer. Methods Sixty cases of gastric cancer from September 2011 to September 2013 of our hospital were as the research object, and compared with 40 cases of benign gastric disease over the same period. These patients＇ venous blood were collected, the CTCs counts were determined by using the CellTracks AutoPrep fluorescence scaning system, and the relationship between CTCs and clinicopathological features of gastric cancer was analyzed. Results The detection rate of CTCs in gastric cancer patients was 70.0% （42/60）, in control group was 7.5% （3/40）. The positive rate of CTCs in peripheral blood of patients with gastric cancer was significantly higher than that of benign gastric disease （P〈0.05）. The positive rate of CTCs in peripheral blood were no correlated with gender, age, N staging, distant metastasis, tumor size, and vascular invasion （P〉0.05）, but were correlated with the TNM staging of tumor and degree of differentiation （P〈0.05）. The cumulative survival rates of 12 months and 18 months after operation in CTCs negtive patients with gastric cancer were higher than that CTCs positive patients （P〈0.05）. Conclusions The detection of CTCs is easy to manage and repeatable. The positive rate of CTCs in gastric patients is higher, which can reflect the progression of tumor and serve as the prognostic index in gastric cance patients.

关 键 词：胃癌 循环肿瘤细胞 免疫磁珠 荧光细胞化学染色 

分 类 号：R735.2[医药卫生—肿瘤]