16SrRNA基因检测在自发性腹膜炎快速诊断中的应用价值  

Application value of 16S rRNA gene detection in the rapid diagnosis of spontaneous peritonitis

作　　者：叶丽君[1] 喻长法[1] 段达荣[1] 任应鹏[1] 张仙森[1] 蒋亚萍[1] 

机构地区：[1]台州市第一人民医院,浙江台州318020

出　　处：《中国卫生检验杂志》2015年第12期1978-1979,1993,共3页Chinese Journal of Health Laboratory Technology

基　　金：浙江省黄岩科技局资助项目(201305926)

摘　　要：目的探讨运用16S rRNA基因检测技术在快速诊断自发性腹膜炎的临床价值。方法针对16S rRNA区域的恒定区,设计一条通用引物,对已知病原菌、人类基因组DNA、巨细胞病毒和白色假丝酵母菌进行PCR扩增,检验方法的特异性,用倍比稀释法检测方法的灵敏性。然后用此方法检测自发性腹膜炎患者的腹水样本,并与培养法进行比较分析。结果已知病原菌均扩增出了特异产物,人类基因组DNA、巨细胞病毒和白色假丝酵母菌无相对应产物,PCR敏感性可达1 pg大肠杆菌DNA。69例腹水样本PCR方法检测阳性率为33.3%,培养法阳性率为14.5%,PCR法阳性率显著高于培养法(P<0.05)。结论 16S rRNA基因检测技术具有检测时间短、敏感性高、结果准确可靠等优点,具有重要的临床应用价值。Objective To explore the clinical value of using 16 S rRNA gene detection technology in rapid the diagnosis of spontaneous peritonitis. Methods A general primer was designed in view of the 16 S rRNA constant region. The known pathogens,human genomic DNA,Cytomegalo virus and Candida albicans were amplified by PCR to test the specificity,and the dilution method was used to test sensitivity,besides,this method was used to detect patients samples with spontaneous peritonitis to confirm infection cases,and to compare it with the culture method. Results The known pathogens were amplified by PCR with specific product,however,human genomic DNA,Cytomegalovirus and Candida albicans were without corresponding product.The PCR sensitivity could reach 1 pg E. coli DNA. The positive rate of PCR method was 33. 3% and the culture method was14. 5%,the positive rate of PCR method was significantly higher than that of culture method（ P〈0. 05）. Conclusion The16 S rRNA gene technology has important applied value with short detection time,high sensitivity,accuracy and reliability.

关 键 词：16SRRNA基因 自发性腹膜炎 快速诊断 

分 类 号：R446.5[医药卫生—诊断学]