妇科肿瘤组织中线粒体DNA4 977 bp缺失及生物学意义  

Mitochondrial DNA 4 977 bp deletion in gynecological tumor tissue and biological significance

作　　者：沈慧[1] 李玉娟[2] 谢静燕[2] 左绪磊[1] 

机构地区：[1]上海复旦大学附属金山医院妇产科,201508 [2]南京市第一医院妇产科

出　　处：《中国妇幼保健》2015年第21期3707-3709,共3页Maternal and Child Health Care of China

基　　金：江苏省南京市卫生局科研基金〔YKK06070〕

摘　　要：目的明确线粒体DNA4 977bp大片段缺失在妇科肿瘤组织及对应患者血清中的频率,为妇科肿瘤的早期临床诊断寻找简便、准确的分子标记。方法运用引物转化PCR的方法对25例妇科恶性肿瘤患者(观察组)、13例癌旁正常组织(对照组)和25例妇科良性肿瘤患者(对照组)的肿瘤组织及对应患者术前血清标本mt DNA 4 977 bp缺失进行分析比较。结果在19例卵巢良性肿瘤中12例有缺失(缺失率为63%),6例子宫肌瘤患者正常内膜均没有缺失(缺失率为0),6例卵巢癌均无缺失(缺失率为0),7例子宫颈癌和癌旁正常组织各3例有缺失(缺失率为43%),6例子宫内膜癌中5例有缺失(缺失率为83%),6例外阴癌癌组织缺失率为100%,癌旁正常组织无缺失。妇科恶性肿瘤患者血清中没有缺失,25例妇科良性肿瘤患者血清中4例有缺失(缺失率为16%)。结论妇科恶性肿瘤中线粒体DNA4977 bp大片段缺失的不同发生率提示检测这种缺失可能会成为区分不同妇科肿瘤的分子标记物。检测妇科肿瘤患者血清mt DNA4 977bp大片段缺失对预测妇科肿瘤的发生没有临产意义。Objective To clarify the frequency of mitochondrial DNA 4 977 bp deletion in gynecological tumor tissue and the matched patient＇s serum, find a simple and accurate molecular marker for early clinical diagnosis of gynecological tumor. Methods Primer- shift PCR method was used to analyze and compare mitoehondrial DNA 4 977 bp deletion in gynecological tumor tissue and the matched patient＇s serum samples in 25 patients with gynecological malignant tumor, 13 patients with matched normal tissue adjacent to tumor, and 25 patients with gynecological benign tumor. Results Among 19 patients with gynecological benign tumor, 12 patients were found with mitoehondrial DNA 4 977 bp deletion, the deletion rate was 63% ; among 6 patients with hysteromyoma and ovarian cancer, no mitochondrial DNA 4 977 bp deletion was found in normal endometrium, the deletion rate was 0% ; among 7 patients with cervical cancer and 7 patients with matched normal tissue adjacent to cervical cancer, 3 patients were found with mitoehondrial DNA 4 977 bp deletion, respectively, the deletion rates were 43%, respectively; among 6 patients with endometrial cancer, 5 patients were found with mitochondrial DNA 4 977 bp deletion, the deletion rate was 83% ; the deletion rate was 100% in tumor tissue of 6 patients with carcinoma of vulva, no mitoehondrial DNA 4 977 bp deletion was found in matched normal tissue adjacent to carcinoma of vulva. No mitoehondrial DNA 4 977 bp deletion was found in serum samples of patients with gynecological malignant tumor; among 25 patients with gynecological benign tumor, 4 patients were found with mitoehondrial DNA 4 977 bp deletion in serum, the deletion rate was 16%. Conclusion The different incidence rates of mito- ehondrial DNA 4 977 bp deletions in gynecological malignant tumors suggest that this deletion may be a molecular marker for distinguishing different gynecological tumors. Detecting mitochondrial DNA 4 977 bp deletion has no clinical significance in predicting the onset of gynecological tumor.

关 键 词：MTDNA 4 977 bp缺失 生殖器肿瘤 

分 类 号：R737.3[医药卫生—肿瘤]