MK-801对6-OHDA诱导PD模型大鼠黑质-纹状体通路作用  

THE PROTECTIVE EFFECT OF MK-801ON NIGROSTRIATAL PROJECTION SYSTEM IN 6-OHDA-INDUCED PARKINSON DISEASE MODELS

作　　者：夏建建 徐华敏[1] 姜宏[1] 谢俊霞[1] 

机构地区：[1]山东省神经相关疾病的机制与防治重点实验室青岛大学

出　　处：《青岛大学医学院学报》2015年第4期379-380,383,共3页Acta Academiae Medicinae Qingdao Universitatis

基　　金：国家自然科学基金项目(81430024;31371081);科技部国家重点基础研究发展计划973计划子课题(2011CB504102)资助项目

摘　　要：目的观察N-甲基-D-天门冬氨酸受体阻滞剂MK-801对6-羟基多巴胺（6-OHDA）诱导的帕金森病（PD）模型大鼠黑质-纹状体通路的作用。方法将40只大鼠随机分为正常对照组、6-OHDA模型组、MK-801低剂量（0.1mg/kg）处理组和MK-801高剂量（0.2mg/kg）处理组。采用6-OHDA单侧损毁大鼠黑质制备PD模型。应用免疫组织化学法检测各组大鼠黑质内酪氨酸羟化酶（TH）免疫阳性神经元数目,高效液相色谱法检测纹状体内多巴胺（DA）的含量。结果与正常对照组相比,6-OHDA模型组大鼠6-OHDA损毁侧中脑黑质TH免疫阳性神经元数目、纹状体内DA含量显著降低（F=478.02、34.86,q=51.25、14.09,P〈0.05）。与6-OHDA模型组相比,MK-801低剂量处理组和MK-801高剂量处理组大鼠黑质TH免疫阳性神经元数目、纹状体内DA含量均显著增加（q=4.27~14.96,P〈0.05）。结论 MK-801对PD模型大鼠黑质-纹状体通路有明显的保护作用。Objective To explore the protective effect of MK-801 on nigrostriatal projection system in 6-hydroxydopamine（6-OHDA）-induced Parkinson disease（PD）models. Methods Forty rats were randomized to control group,6-OHDAtreated group,0.1mg/kg MK-801 pretreated group and 0.2mg/kg MK-801 pretreated group.6-OHDA was used to create PD rat models.Applying immunohistochemical method and high performance liquid chromatography to detect the number of tyrosine hydroxylase（TH）-positive neurons in the substantia nigra（SN）and dopamine contents in the striatum of rats,respectively. Results The number of TH-positive neurons in the SN and dopamine contents in the striatum decreased significantly in 6-OHDAtreated rats compared with the controls（F=478.02,34.86;q=51.25,14.09;P0.05）.Compared with 6-OHDA-treated group,the numbers of TH-positive neurons and striatal DA contents in low-dose-treated and high-dose-treated groups obviously increased（q= 4.27-14.96,P0.05）. Conclusion MK-801 shows a notable protective effect on nigrostriatal path action in rat models of 6-OHDA-induced PD.

关 键 词：地卓西平马来酸盐 帕金森病 受体 N-甲基-D-天冬氨酸 多巴胺 

分 类 号：R742.5[医药卫生—神经病学与精神病学]