细胞融合在多发性骨髓瘤骨病发病机制中的作用探讨  

Investigation of the pathogenesis of cell fusion in multiple myeloma bone disease

作　　者：周敏[1] 傅晋翔[1] 王子妍[1] 袁育青[1] 居颂光[1] 张立莹[1] 

机构地区：[1]苏州大学附属第二医院血液科,215004

出　　处：《白血病．淋巴瘤》2015年第6期324-327,共4页Journal of Leukemia & Lymphoma

基　　金：国家自然科学基金（81272631）

摘　　要：目的 探讨人骨髓基质细胞与人骨髓瘤细胞株RPMI 8226的融合细胞在多发性骨髓瘤骨病发病过程中可能的机制.方法 细胞示踪绿色荧光探针（CMFDA）及红色荧光探针（CMTMR）分另别标记的细胞通过化学促融剂聚乙二醇（PEG-1000）诱导融合,建立细胞融合模型.染色体核型分析,确定融合细胞是否发生细胞核的融合;鉴定融合细胞干性基因及促融相关性基因SIRPα、DC-STAMP的表达情况.结果 PEG-1000能够介导骨髓基质细胞与RPMI 8226细胞融合;超过80％的融合细胞染色体数目在80条左右;融合细胞不仅表现出骨髓基质细胞的特征及干性相关基因c-myc、Klf-4、OCT-4表达阳性,而且融合相关性基因SIRP α及DC-STAMP也表达阳性.结论 骨髓基质细胞与RPMI 8226细胞间可形成融合细胞,融合细胞具有进一步成融的潜力,可能是促进多发性骨髓瘤骨破坏的重要原因之一.Objective To discuss function of the fusion cells of human bone marrow stromal cells （BMSCs） and human myeloma cell RPMI 8226 in the pathogenesis of multiple myeloma bone disease.Methods The cells,labeled by cell tracer green fluorescent probe （CMFDA） and red fluorescent probe （CMTMR）,respectively,were induced into fusion by chemical polyethylene glycol （polyethyleneglycol,PEG-1000）,and cell fusion model was set up.Whether fusion cells had nucleus fusion was determined by Karyotype analysis.The expressions of stemness-related genes,SIRP α gene and DC-STAMP gene in fusion cells were identified.Results Polyethylene glycol （PEG-1000） could mediate the integration of BMSCs and RPMI 8226 cells.The number of chromosomes in more than 80 % the hybrid cells was about 80.Fusion cells not only showed that BMSCs,stemness-related genes of c-myc,Klf-4 and OCT-4 genes expressed positively,but also the fusion-related genes SIRPα and DC-STAMP expressed positively.Conclusion BMSCs and RPMI 8226 cells can form fusion cells,and the cells have the potential for further integration,which is one of the important reasons for the promotion of muhiple myeloma bone destruction.

关 键 词：细胞融合 多发性骨髓瘤 骨髓瘤骨病 聚乙二醇 

分 类 号：R733.3[医药卫生—肿瘤]