海兔素对脑胶质瘤异位移植瘤的抑制作用  

INHIBITORY EFFECT OF APLYSIN ON GLIOMA HETEROTOPIC TRANSPLANTATION TUMOR

作　　者：马磊[1,2] 梁惠[1] 宫安静[3] 

机构地区：[1]青岛大学公共卫生学院,山东青岛266021 [2]青岛市城阳区人民医院感染科 [3]青岛大学附属医院神经外科

出　　处：《齐鲁医学杂志》2015年第4期423-425,429,共4页Medical Journal of Qilu

基　　金：产学研合作引导计划:应用基础研究(10-3-4-3-11-jch)

摘　　要：目的通过观察海兔素对小鼠脑胶质瘤异位移植瘤血管内皮生长因子（VEGF）、表皮生长因子受体（EGFR）及环氧化酶-2（COX-2）表达影响,探讨其对脑胶质瘤的抑制作用。方法将75只昆明种小鼠于左前腋下皮下接种1×10^9/L胶质瘤细胞悬液,24h后随机分为5组,每组15只。脑胶质瘤异位移植瘤模型组（A组）小鼠以等体积大豆色拉油灌胃,海兔素低剂量组（B组）以50 mg/（kg·d）剂量海兔素灌胃,海兔素中剂量组（C组）以100mg/（kg·d）剂量海兔素灌胃,海兔素高剂量组（D组）以150mg/（kg·d）剂量海兔素灌胃,替莫唑胺（TMZ）阳性对照组（E组）以20mg/（kg·d）剂量TMZ灌胃,每天灌胃1次,连续给药2周。末次灌胃24h后,称体质量,经眼球取血后处死小鼠。无菌条件下剥离肿瘤组织,免疫组化法测定肿瘤组织中VEGF、EGFR及COX-2的表达情况。结果与A组比较,B、C、D组随着海兔素剂量的增加,VEGF、EGFR、COX-2的表达均有逐渐降低趋势,各组间比较,除D组与E组差异无显著性外,其他各组间差异均有显著性（H=22.21~24.69,U=4.50~16.00,P〈0.05）。结论海兔素可抑制小鼠脑胶质瘤异位移植瘤生长,其作用机制可能与抑制小鼠脑胶质瘤异位移植瘤组织VEGF、EGFR及COX-2的表达有关。Objective To observe the inhibition of aplysin on the expressions of VEGF,EGFR and COX-2in glioma heterotopic transplanted tumor（GHTT）in mice. Methods Seventy-five Kunming mice were given subcutaneous inoculation of1×10^9 cell/L glioma-cell suspension on left armpit to create a GHTT model in mice and evenly randomized to five groups-group A（GHTT model group）,the mice in this group were given equal-volume soybean salad oil;group B（low-dose aplysin group）,aplysin 50mg/（kg·d）;group C（medium-dose aplysin group）,100 mg/（kg·d）aplysin;group D（high-dose aplysin group）,150mg/（kg·d）aplysin;group E（temo-zolomide group）,temo-zolomide（TMZ）20mg/（kg·d）,all were given intragastrically,once a day for two weeks.After 24 hof the last gavage,the weight of mice was measured,blood was taken from eyeball.Tumors were dissected under sterile condition.The expressions of VEGF,EGFR and COX-2in tumor tissue were detected using immunohistochemical method. Results Compared with group A,the expressions of VEGF,EGFR and COX-2in groups B,C and D showed a gradual decline along with the increase of aplysin dose,the differences among each groups were significant,except that between groups D and E（H =22.21-24.69,U=4.50-16.00,P〈0.05）. Conclusion Aplysin can inhibit the growth of glioma heterotopic transplantation tumor,its mechanism is likely to be associated with restraining the expressions of VEGF,EGFR and COX-2in mouse＇s glioma.

关 键 词：海兔素 神经胶质瘤 血管内皮生长因子类 受体 表皮生长因子 环氧化酶2 

分 类 号：R739.4[医药卫生—肿瘤]