白细胞介素-6诱导CD44+ CD24-/low肿瘤干细胞生成促进乳腺癌MCF7细胞辐射抵抗的研究  

Interleukin-6 promotesradiation resistance of breast cancer MCF7 cellsby induction of CD44+ CD24-/low cancer stem cells

作　　者：郭昭泽[1] 刘民锋[1] 詹军芳[1] 谢国柱[1] 叶长生[1] 

机构地区：[1]南方医科大学南方医院乳腺科

出　　处：《临床外科杂志》2015年第6期431-434,共4页Journal of Clinical Surgery

基　　金：国家自然科学基金资助项目(81302326)

摘　　要：目的：上皮-间充质转化（EMT）能诱导 CD44＋CD24－／low乳腺癌干细胞的产生，白细胞介素-6（IL-6）是很强的 EMT 诱导因子，探讨 IL-6是否通过诱导 CD44＋CD24－／low细胞的产生，从而促进乳腺癌 MCF7细胞的辐射抵抗。方法含50 ng／ml IL-6的培养基培养 MCF7细胞，流式细胞术检测 CD44＋CD24－／low乳腺癌干细胞标记的变化，实时定量聚合酶链反应（Real-time PCR）及Western blot 检测 EMT 标记分子的表达水平，进一步利用流式细胞术从诱导组中分选出 CD44＋CD24－／low细胞，克隆形成实验检测分选后细胞的辐射抵抗能力。结果IL-6能诱导 MCF7细胞发生间充质样转化，诱导5 d 后 CD44＋CD24－／low细胞显著富集，诱导形成的 CD44＋CD24－／low细胞的辐射抵抗能力显著提高。结论IL-6诱导了 CD44＋CD24－／low 乳腺癌干细胞的产生，从而促进MCF7细胞的辐射抵抗。Objective To determine whether interleukin-6（IL-6）,a welly established potent in-ducer of epithelial-mesenchymal transition（EMT）,promotes radiation resistance of breast cancer MCF7 cellsby induction of CD44 ＋CD24 -/low cancer stem cells Methods Breast cancer MCF-7 cells were cul-tured in 1 0% FBS DMEMwith 50 ng/ml IL-6.The percentages of CD44 ＋CD24 -/low cells were measured by flow cytometry.Real-time RT-PCR and western blotting were used to determine expression of markers associated with EMT.The capacity of radiation resistance was tested by colony formation.Results IL-6 induced EMT of MCF7 cells.CD44 ＋CD24 -/low cells were enriched after 5-day induction of IL-6 and these induced cells exhibited enhancedradiation resistance.Conclusion IL-6 induced the generation of CD44 ＋CD24 -/low cells and promoted radiation resistance in breast cancer MCF7 cells.

关 键 词：白细胞介素-6 上皮-间充质转化 干细胞 辐射抵抗 

分 类 号：R737.9[医药卫生—肿瘤]