3种石油烃降解菌对石油烃的降解效果及其细胞表面疏水性  

Degradation effect and cell hydrophobicity of the three kinds of petroleum hydrocarbon degrading strains

作　　者：刘虹[1] 刘娜[2] 吕静[1] 温钢[3] 付净[1] 

机构地区：[1]吉林化工学院资源与环境工程学院,吉林吉林132022 [2]吉林大学地下水资源与环境教育部重点实验室,吉林长春130021 [3]吉林化工学院生物与食品工程学院,吉林吉林132022

出　　处：《环境污染与防治》2015年第7期13-17,22,共6页Environmental Pollution & Control

基　　金：国家自然科学基金资助项目(No.41302185)

摘　　要：选取3种石油烃降解菌:假单胞菌(Pseudomonas sp.,DS-1)、铜绿色假单胞菌(Pseudomonas aeruginosa,DS-2)和无色杆菌(Achromobacter sp.,DS-3),研究其对石油烃的降解效果及其细胞表面疏水性。结果表明,经过6d的降解,3种石油烃降解菌对石油烃的降解率分别为99.08%、79.75%、84.34%。石油烃的黏附性测试和盐析聚集测试结果表明,3种石油烃降解菌均表现出较高的细胞表面疏水性,其规律为DS-1>DS-3>DS-2。其中DS-1的细胞表面疏水性最高,达65.90%。DS-1、DS-2和DS-3菌株发生盐析聚集所需最小(NH4)2SO4摩尔浓度分别为2.0、2.8、2.4 mol/L。菌株的细胞表面疏水性和降解有机物的能力有着较高的相关性。Three kinds of petroleum hydrocarbon degrading strains Pseudomonas sp.,Pseudomonas aeruginosa and Achromobacter sp. were selected. The degradation effect and ceils hydrophobicity of them were studied. The results showed that degradation rate of three kinds of petroleum hydrocarbon degrading strains were 99.08M, 79.75 % and 84.34 % respectively after six days of degradation. Both hydrocarbon adhesion test and salt aggregation test indicated that all the three strains exhibited good hydrophobicity. The cell surface hydrophobicity of the three strains was DS-1〉DS-3〉DS-2.The cell surface hydrophobicity of DS-1 was the highest,which reached up to 65.90%. The minimum （NH4）2SO4 concentrations required to gather were 2.0 mol/L, 2.8 mol/L and 2.4 mol/L for DS-1, DS-2 and DS-3. It proved that the surface hydrophobicity of the strain and its degrading ability for organic pollutants had higher correlation.

关 键 词：石油烃 降解菌 降解效果 细胞表面疏水性 

分 类 号：X172[环境科学与工程—环境科学]