基于DPPH、FRAP法的薄荷药材抗氧化谱效关系研究  

Spectrum-effect relation in antioxidant activity of Menthae haplocalycis Herba based on DPPH and FRAP assay

作　　者：徐晶晶[1] 刘斌[1] 

机构地区：[1]北京中医药大学中药学院,北京100102

出　　处：《北京中医药大学学报》2015年第6期405-410,共6页Journal of Beijing University of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(No.81173520);北京中医药大学自主选题资助项目(No.2014-JYBZZ-XS-077)

摘　　要：目的通过研究薄荷药材特征图谱与其抗氧化活性的相关关系,探讨薄荷抗氧化活性物质基础。方法采用1,1-二苯基-2-三硝基苯肼(DPPH)、总抗氧化能力评估法(FRAP)法分别测定薄荷样品抗氧化活性,同时测定样品相应的特征图谱,通过偏最小二乘法研究特征图谱色谱峰和抗氧化活性相关性,筛选活性色谱峰,并结合HPLC-MS/MSn进行结构鉴定。结果筛选得到的橙皮苷、香叶木苷、蒙花苷、百里香新、黄姜味草酸等6个化合物与清除DPPH自由基能力相关,橙皮苷、蒙花苷、反式丹酚酸J、迷迭香酸等8个化合物与总抗氧化能力相关。结论谱效关系研究能够表征中药化学成分-药效相关关系,可以较为快捷地从复杂体系中筛选活性化合物。Objective To explore the active ingredients of Menthae Haplocalycis Herba by investigating the correlation between HPLC spectrum and antioxidant activity. Methods We established DPPH[1,1-Diphenyl-2-picrylhydrazyl radical 2,2-Diphenyl-1-（ 2,4,6-trinitrophenyl） hydrazyl,DPPH] and FRAP（ ferric ion reducing antioxidant power,FRAP） assay to determine the antioxidant activity of Menthae Haplocalycis Herba. The spectrum-effect correlation was studied by using PLS（ Partial least squares）.HPLC-MS / MSnwas applied to identify their structures. Results Six compounds including hesperidin,diosmin,buddleoside, thymusin and xanthomicrol, which made great contributions to the DPPH scavenging activity,were screened; eight compounds,including hesperidin,buddleoside,rosmarinic acid and trans Salvianolic acid J,were obtained which made great contributions to the total antioxidant activity. Conclusion It is an effective way to characterize spectrum-efficacy correlation and clarify the active components of Chinese medicinal.

关 键 词：薄荷 抗氧化活性 谱效关系 1 1-二苯基地-2-三硝基苯肼 总抗氧化能力评估法 偏最小二乘法 

分 类 号：R284.1[医药卫生—中药学]