血管新生相关因子在乙型肝炎相关肝细胞癌各临床分期中的表达分析  

Angiogenesis-related factors in various clinical stages of hepatitis B-related hepatocellular carcinoma

作　　者：韩珍[1] 孙焕芹[1] 江娜[1] 刘宁[1] 徐杰[1] 孙坚萍[1] 张永宏[1] 

机构地区：[1]首都医科大学附属北京佑安医院,北京100069

出　　处：《临床肝胆病杂志》2015年第6期932-935,共4页Journal of Clinical Hepatology

基　　金：北京市科技计划课题(D131100005313004;D131100005313005);国家国际科技合作专项项目(2012DFA30850)

摘　　要：目的研究血管新生相关因子在乙型肝炎相关肝细胞癌各临床分期中的表达情况及临床意义。方法选取2011-2013年于北京佑安医院就诊的乙型肝炎相关肝癌患者70例,按照肝癌分期标准将其分为肝癌早早期(n=18)、肝癌早期(n=17)、肝癌进展期(n=18)和肝癌晚期(n=17),另选取18例健康人群作为对照。运用Luminex 200液相悬浮芯片系统检测血管内皮生长因子(VEGF)、血管内皮细胞生长因子受体(VEGFR)1和VEGFR-2、肝细胞生长因子(HGF)在乙型肝炎相关肝癌各临床分期中的表达情况。计量资料组间比较采用t检验。结果与对照组相比,在肝癌早早期,VEGF和HGF水平明显升高,差异均有统计学意义(t值分别为2.69、2.18,P值分别为0.008、0.002);在肝癌早期,VEGF、VEGFR-1和HGF水平均明显升高,差异具有统计学意义(t值分别为1.73、2.03、2.54,P值分别为0.01、0.009、0.008);在肝癌进展期,VEGF、VEGFR-1和HGF水平均明显升高,差异具有统计学意义(t值分别为1.64、4.38、4.02,P值分别为0.01、0.007、0.005);在肝癌晚期,VEGF、VEGFR-1、VEGFR-2和HGF水平均明显升高,差异具有统计学意义(t值分别为4.37、20.24、2.21、3.06,P值分别为0.007、0.009、0.01、0.003)。结论在肝癌早早期,血管新生相关因子VEGF和HGF已明显升高,随着病情进展VEGFR-1和VEGFR-2的表达也明显升高。Objective To investigate the expression levels of angiogenesis- related factors in various clinical stages of hepatitis B- related hepatocellular carcinoma（ HCC） and their clinical significance. Methods Seventy patients with hepatitis B- related HCC who were admitted to our hospital from 2011 to 2013 were enrolled as subjects and divided into four groups according to the HCC staging criteria： very early HCC group（ n = 18）,early HCC group（ n = 17）,advanced HCC group（ n = 18）,and end- stage HCC group（ n = 17）. Eighteen healthy volunteers were used as controls. The Luminex 200 liquid suspension array system was used to determine the expression levels of vascular endothelial growth factor（ VEGF）,vascular endothelial growth factor receptors（ VEGFR- 1 and VEGFR- 2）,and hepatocyte growth factor（ HGF） in each clinical stage of hepatitis B- related HCC. Comparison of continuous data between two groups was made by t test. Results Compared with the control group,the very early HCC group had significantly higher levels of VEGF and HGF（ t = 2. 69,P = 0. 008; t =2. 18,P = 0. 002）. The early HCC group had significantly higher levels of VEGF,VEGFR- 1,and HGF than the control group（ t = 1. 73,P = 0. 01; t = 2. 03,P = 0. 009; t = 2. 54,P = 0. 008）. The levels of VEGF,VEGFR- 1,and HGF were significantly higher in the advanced HCC group than in the control group（ t = 1. 64,P = 0. 01; t = 4. 38,P = 0. 007; t = 4. 02,P = 0. 005）. In the end- stage HCC group,the levels of VEGF,VEGFR- 1,VEGFR- 2,and HGF were significantly higher than those in the control group（ t = 4. 37,P =0. 007; t = 20. 24,P = 0. 009; t = 2. 21,P = 0. 01; t = 3. 06,P = 0. 003）. Conclusion The levels of angiogenesis- related factors,VEGF and HGF,have already been substantially increased in the very early HCC,which induces a substantial upregulation of expression of VEGFR- 1 and VEGFR- 2 along with the progression of HCC.

关 键 词：癌 肝细胞 肝炎 乙型 血管内皮生长因子类 血管内皮生长因子受体1 血管内皮生长因子受体2 肝细胞生长因子 

分 类 号：R735.7[医药卫生—肿瘤]