HGF/PARP-1信号通路调控卵巢癌细胞侵袭转移的作用  

Mechanism of HGF/PARP-1 signaling pathway regulating invasion and metastasis of ovarian cancer

作　　者：吕树卿[1] 尉蔚[1] 张灿灿[1] 田永杰[1] 

机构地区：[1]山东大学附属省立医院妇产科,山东济南250021

出　　处：《山东大学学报（医学版）》2015年第7期1-7,共7页Journal of Shandong University:Health Sciences

基　　金：国家自然科学基金应急管理项目(81441075);山东省自然科学基金(ZR2014HM108);山东省自然科学基金青年项目(ZR2013HQ030)

摘　　要：目的观察聚腺苷酸二磷酸核糖聚合酶-1(PARP-1)在肝细胞生长因子(HGF)诱导的卵巢癌细胞侵袭转移中的作用及相关信号转导通路。方法 Transwell实验检测不同浓度HGF对卵巢癌SKOV-3细胞体外侵袭力的影响;实时荧光定量PCR、Western blotting检测不同浓度及作用时间HGF对SKOV-3细胞PARP-1m RNA及蛋白表达的影响;RNA干扰沉默SKOV-3细胞的PARP-1基因,按不同处理因素将SKOV-3细胞分为空白对照组、HGF组、PARP-si RNA组、HGF+PARP-si RNA组;NC-si RNA组、HGF+NC-si RNA组。Western blotting检测不同处理组细胞内PARP-1蛋白表达。Transwell实验检测各组细胞侵袭力。ELISA法检测各组细胞上清中基质金属蛋白酶-2(MMP-2)的含量。结果 HGF可明显促进SKOV-3细胞的体外侵袭能力,并呈浓度时间依赖性。40 ng/m L HGF作用24 h后,HGF可明显上调SKOV-3细胞中PARP-1的表达水平;PARP-si RNA组细胞PARP-1的表达低于NC-si RNA组(P<0.05),干扰PARP-1的表达降低了HGF对SKOV-3细胞侵袭力及M M P-2含量的影响。结论HGF可促进卵巢癌SKOV-3细胞的侵袭转移,其作用机制可能与PARP-1介导的M M P-2表达增加有关。Objective To investigate the effects and signaling pathway of HGF-regulated PARP-1 expression on invasion and metastasis of ovarian cancer. Methods SKOV-3 cells were incubated with various concentrations of HGF to examine their invasive abilities by Transwell assay. Relative expression of PARP-1 after HGF treatment in SKOV-3 cells was analyzed by RT-PCR and Western blotting. SKOV3 cells were transfected with either negative control si RNA（ NC-si RNA group） or PARP-1 si RNA（ PARP1-si RNA group）,and divided into different groups depending on different factors：blank control group; HGF group; PARP-si RNA group; HGF ＋ PARP-si RNA group; NC-si RNA group; HGF ＋NC-si RNA group. The expression of PARP-1 in different groups was measured with Western blotting. The invasive abilities were examined with Transwell assay. The expression of M M P-2 was detected with ELISA. Results HGF promoted cell invasion in a concentration- and time-dependent manner in the SKOV-3 cell line. The expression of PARP-1 in SKOV-3 cells increased after HGF 40 ng / m L treatment for 24 hours. The expression of PARP-1 in PARP1-si RNA group was lower than that in NC-si RNA group（ P〈0. 05）. The transfection significantly reduced impacts of HGF on SKOV-3cell invasion and the expression of M M P-2. Conclusion HGF promotes the invasion and metastasis of ovarian cancer.This effect may be related to the increased expression of M M P-2 which is mediated by PARP-1.

关 键 词：卵巢肿瘤 肝细胞生长因子 侵袭 聚腺苷酸二磷酸核糖聚合酶-1 基质金属蛋白酶-2 

分 类 号：R737.31[医药卫生—肿瘤]