bFGF经鼻给药对大鼠缺血再灌注脑损伤的保护作用  

Neruoprotective effect of bFGF on ischemic-reperfusion injury in SD rats via intranasal administration

作　　者：姜义娜[1] 田福荣 林倩[1] 姚锐[2] 赵应征[1] 

机构地区：[1]温州医科大学药学院,浙江温州325035 [2]北京老年医院医保办,北京100095

出　　处：《温州医学院学报》2015年第8期560-565,共6页Journal of Wenzhou Medical College

基　　金：国家自然科学基金资助项目(81272160)

摘　　要：目的:探索碱性成纤维细胞生长因子(b FGF)经鼻给药这一新途径对脑缺血神经元的保护作用。方法:用颈外动脉线栓法(ECA法)制备大脑中动脉栓塞的局灶性脑缺血再灌注(I/R)动物模型,于术后24 h进行神经功能评分检验建模成功与否。对造模成功大鼠经鼻给予b FGF治疗后,分别通过神经功能评分、TTC染色测定脑梗死体积、HE染色以及免疫组织化学法来评价其疗效。结果:大鼠经b FGF治疗后,脑梗死体积显著减少(P<0.05);染色结果显示治疗后大鼠的神经细胞数目增加,并且p-Akt阳性细胞表达量增加。结论:b FGF通过鼻腔给药这一新途径能够显著减少缺血脑组织神经细胞的死亡,对缺血神经元具有较好的保护作用。Objective：To investigate the neuroprotective effects of b FGF（basic fibroblast growth factor） via intranasal administration for ischemic neurons of brain.Methods：The transient focal cerebral ischemia-reperfusion model of adult male SD rats was established with a method of line-plugging to the external carotid artery.Twenty-four hours after the reperfusion,behavioral test（neurologic deficit scoring） was carried out to evaluate the neurological deficiency.The neuroprotective effects of b FGF via intranasal administration to the model rats were evaluated with neurologic deficit score,infarct volume,histology and immunohistochemistry staining.Results：The transient focal cerebral ischemia-reperfusion model were established successfully.Comparing with the ischemia-reperfusion group,the level of infarction volume was significantly decreased（P〈0.05）,the number of nerve cells were increased and the expression of p-Akt positive cells were significantly up-regulated via intranasal administration of b FGF group.Conclusion：By the new pathway of nasal administration,b FGF can decrease the infarction volume and protect neurons from cerebral ischemic injury.

关 键 词：碱性成纤维细胞生长因子 缺血再灌注脑损伤 颈外动脉线栓法 鼻腔给药 大鼠 

分 类 号：R743.3[医药卫生—神经病学与精神病学]