不同品种大豆叶片表皮蜡质提取后的扫描电镜观察  

SEM Observation after Extraction of Epicuticular Wax on Soybean Leaves

作　　者：李娜[1] 王丽娜[1] 金勋[1] 芮海英[1] 潘红丽[1] 金铃[1] 李泽宇[1] 于吉东[1] 

机构地区：[1]黑龙江省农业科学院大庆分院,黑龙江大庆163316

出　　处：《大豆科学》2015年第3期540-544,共5页Soybean Science

摘　　要：以抗线11和黑农48两个大豆品种的成熟叶片为材料,用氯仿和正己烷两种溶剂,采用常温法和加热法提取大豆叶片表皮蜡质,对脱蜡后的叶片进行扫描电镜观察。结果显示加热法效果好于常温法。采用常温法,两种溶剂都不能将大豆叶片表皮蜡质提取干净,仍有少量絮状或颗粒状蜡质残留,但氯仿的提取效果好于正己烷;采用加热法,两种溶剂不同处理时间均能将叶片表皮蜡质完全提取干净,处理时间对蜡质的提取结果有明显影响,加热30 s时叶片表皮细胞均发生不同程度的变形。通过扫描电镜观察得出以氯仿为溶剂,加热60℃、处理时间15 s为大豆叶片简单可行的脱蜡方法。Extraction of epicuticular wax on mature soybean leaves（ Kangxian 11 and Heinong 48） are studied comparatively with solution CHCl3 and solution n-hexane with room-temperature method and high-temperature method. The microstructure observed by scanning electronic microscope（ SEM） showed that high-temperature method was better than room-temperature method. Epicuticular wax was not completely extracted with room temperature method with both solution CHCl3 and solution nhexane,there was still a small amount of flocculent or granular waxy residue,but the extraction effect of chloroform was better than n-hexane. Epicuticular wax was completely extracted with high-temperature method with both solution CHCl3 and solution n-hexane,extraction time was found significantly affecting the extracted wax quality,high-temperature treatment 30 s,leaf epidermal cells had undergone varying degrees of deformation. In the present study,60℃,15 s with solution CHCl3 was the effective and simple extraction method for epicuticular wax on soybean leaves by SEM.

关 键 词：大豆 表皮蜡质 氯仿 正己烷 扫描电镜 

分 类 号：S565.1[农业科学—作物学]