紫外诱变选育高效降解甲醛菌株及其降解特性  

Ultraviolet Mutagenesis of Paecilomyces variotii for Formaldehyde Degradation and Degradation Characteristics of Its Mutant Strain

作　　者：姚璐晔[1] 李想[2] 邢鋆 高杏[1] 曹叶萍 顾佳佳[1] 

机构地区：[1]常熟理工学院生物与食品工程学院,江苏常熟215500 [2]江南大学生物工程学院,江苏无锡214122

出　　处：《食品科学》2015年第13期143-147,共5页Food Science

基　　金：2012年全国大学生创新创业实践计划项目(201210333014);常熟理工学院校青年教师科研启动项目(KYZ2011178Z);常熟理工学院2014届本科毕业论文重点资助项目(LG79)

摘　　要：以甲醛降解菌——拟青霉菌(Paecilomyces variotii)CSLG1为出发菌株,对其进行紫外诱变,并考察突变株对甲醛的降解特性。选用功率15 W的紫外灯、照射距离30 cm、照射时间30 s、致死率为88%的诱变强度对出发菌株进行紫外诱变;通过初筛、复筛选育出一株甲醛抗性及其降解能力明显提高的诱变菌株F1-23。结果表明:F1-23临界甲醛抗性质量浓度为7.88 g/L,比诱变前提高22.2%;甲醛脱氢酶酶活力为83.6 U/mg,提高了23.8%。连续传代5代,诱变菌株F1-23的甲醛抗性和降解力基本不变,证实其具有良好的遗传稳定性。诱变菌株F1-23对甲醛的抗性及降解能力均较出发菌株高。Paecilomyces variotii CSLG1 A,a strain able to degrade formaldehyde,was mutagenized by ultraviolet（UV） irradiation and the selected mutants were tested for their abilities to degrade formaldehyde.The UV mutagenesis was carried out by placing the original strain at a distance of 30 cm away from a 15-W UV light lamp for 30 s,yielding a mortality rate of 88%.The mutant stain F1-23 with significantly increased formaldehyde degradation ability was obtained by two rounds of screening.The critical formaldehyde concentration degraded by the mutant strain was 7.88 g/L,which was enhanced by 22.2% when compared with that obtain with the original strain.The activity of formaldehyde dehydrogenase produced by F1-23 was 83.6 U/mg,representing a 23.8% increase over that produced by the original strain.The formaldehyde resistance and degradation capacity of F1-23 remained substantially unchanged after 5 passages,suggesting good genetic stability.In summary,the strain F1-23 holds great promise for application in the exploration of formaldehyde biodegradation.

关 键 词：甲醛降解 拟青霉菌 紫外诱变 甲醛脱氢酶 降解率 

分 类 号：Q939.9[生物学—微生物学]