繁茂膜海绵清除大肠杆菌的过程  

Study on the removal process of Escherichia coli by sponge Hymeniacidon perlevis

作　　者：张喜昌[1,2] 张卫[2] 褚亚东[2] 赵宗宝[2] 谭海东[2] 梁玉波[3] 许淑芬 冷晓飞 李丹 

机构地区：[1]大连海宝渔业有限公司,辽宁大连116045 [2]中国科学院大连化学物理研究所,辽宁大连116023 [3]国家海洋环境监测中心,辽宁大连116023

出　　处：《海洋科学》2015年第5期21-27,共7页Marine Sciences

基　　金：国家海洋"863"高科技研究发展计划项目(2003AA620404)

摘　　要：通过分析海绵清除大肠杆菌的过程,研究海绵净化细菌的机理。作者利用荧光显微镜和激光共聚焦显微镜观测等手段,监测和分析了绿色荧光大肠杆菌(Escherichia coli)在繁茂膜海绵(Hymeniacidon perlevis)体内、体外水环境中数量变化过程。在1 L含有3×107个/m L绿色荧光大肠杆菌的海水中放入鲜重(1.02±0.11)g的繁茂膜海绵24块,处理7 h,海水中的荧光大肠杆菌数量逐渐降低;而海绵体内荧光大肠杆菌数量在2 h时内逐渐增多,之后的2 h趋于稳定,4 h以后开始逐渐减少。水体中大肠杆菌不仅进入海绵体内,而且进入海绵细胞内。含有荧光大肠杆菌的海绵块转入无菌海水中后,海绵体内及细胞中大肠杆菌逐渐消失,而且大肠杆菌没有被释放到环境海水中。分析表明,繁茂膜海绵能够以摄食的方式净化水环境中的大肠杆菌。To investigate the mechanism of sponge on bioremediation of bacteria, the removal process of Escherichia coli by sponge Hymeniacidon perlevis was explored based on analysis of the changing trend of green fluorescent E. coli quantity in water environment and in sponge H. perlevis by means of fluorescent microscope image, confocal microscope image, and other methods. 24 sponge cutting blocks weighted（1.02±0.11）g were put into 1 L seawater containing green fluorescent E. coli at a density of 3×107 cells/m L for 7 h. During the test period, the quantity of E. coli in seawater decreased continuously, while the quantity of E. coli in sponge cuts increased for the first 2 h, remained nearly unchanged for another 2 h, and then decreased gradually. The fluorescent E. coli in seawater was not only in the body of sponge H. perlevis, but also in the cells of sponge. When the sponge cuts containing E. coli were transferred to axenic seawater, the fluorescent E. coli in sponge cells or sponge body disappeared gradually, and the fluorescent E. coli was not released into the water environment. These findings show that sponge H. perlevis can remove E. coli in seawater by means of ingestion and digestion.

关 键 词：繁茂膜海绵(Hymeniacidon perlevis) 绿色荧光大肠杆菌 海绵细胞 净化 

分 类 号：S965.3[农业科学—水产养殖]