重组高效复合干扰素对人肺腺癌A549细胞增殖和凋亡的作用  

Effect of Recombinant Super-compound Interferon(rSIFN-co)on Proliferation and Apoptosis of Human Pulmonary Adenocarcinoma Cell A549

作　　者：阚奇伟[1,2] 刘伦旭[1] 魏光文 蒲强[1] 诸葛雪朋[1] 马林[1] 马军亮[1] 

机构地区：[1]四川大学华西医院胸外科,成都610041 [2]眉山市人民医院胸外科 [3]四川辉阳生命工程股份有限公司,成都610041

出　　处：《四川大学学报（医学版）》2015年第4期508-512,共5页Journal of Sichuan University(Medical Sciences)

基　　金：国家科技重大专项-重大新药创制(No.2009ZX09102-246)资助;四川省科技厅科技支撑计划项目(No.2009SZ0152;No.2011SZ0111)资助

摘　　要：目的研究重组高效复合干扰素（rSIFN-co）对肺腺癌细胞株A549增殖和凋亡的影响。方法筛选rSIFN-co对A549细胞的有效浓度,及与顺铂联合用药的最佳浓度和作用时间。分为rSIFN-co（5μg/mL）组、传统重组集成干扰素α（干复津,5μg/mL）组、rSIFN-co（5μg/mL）＋顺铂（2μg/mL）组、干复津（5μg/mL）＋顺铂（2μg/mL）组、顺铂（2μg/mL）组和RPMI-1640液空白组,分别干预A549细胞。MTT法检测各组7d活细胞数并作生存曲线,流式细胞仪检测48h各组细胞凋亡率,免疫荧光法检测48h各组凋亡相关蛋白Fas、Bcl-2表达。结果 rSIFN-co的有效浓度为1-64μg/mL,顺铂的最低有效浓度为2μg/mL,与2μg/mL顺铂联合用药时rSIFN-co的最佳浓度为5μg/mL,最佳作用时间为48h。生存曲线示rSIFN-co对A549的抑制作用强于干复津,rSIFN-co＋顺铂的抑制作用强于干复津＋顺铂。rSIFN-co可诱导A549细胞凋亡,rSIFN-co组凋亡率高于干复津组（P=0.000）,rSIFN-co＋顺铂组凋亡率高于rSIFN-co组（P=0.004）及顺铂组（P=0.023）。rSIFN-co能增强A549中Fas的表达,并抑制Bcl-2的表达,其中rSIFN-co组Bcl-2表达低于干复津组（P〈0.05）。结论 rSIFN-co能抑制A549增殖,且作用强于干复津,与顺铂联合应用的抑制作用增强,其对A549的抑制作用与调节凋亡相关基因表达有关。Objective To investigate the effect of recombinant super-compound interferon（rSIFN-co）on the proliferation and apoptosis of pulmonary adenocarcinoma cell line A549.Methods Screening tests were conducted to determine the concentrations of rSIFN-co that have a significant impact on A549 and the optimal concentration and duration for the test of rSIFN-co combined with Cisplatin.A549 cells were treated with rSIFN-co,Infergen,rSIFNco＋Cisplatin,Infergen＋Cisplatin,and Cisplatin,respectively,and compared with those cultured in normal medium.The viable A549 cells from Day 1to Day 7were detected by MTT assay.Cell apoptosis was detected by flow cytometry（FCM）.Apoptosis-associated proteins,Fas and Bcl-2 were detected by immunofluoroscence at48 h.Results Effective concentrations of rSIFN-co ranged from 1to 64μg/mL,and a minimal of 2μg/mL Cisplatin was needed.The optimal test condition was set at 5μg/mL rSIFN-co combined with 2μg/mL Cisplatin for a duration of 48 h.rSIFN-co demonstrated a stronger inhibiting effect on cell proliferation than Infergen.The inhibiting efficiency of rSIFN-co＋Cisplatin was also stronger than that of Infergen＋Cisplatin.Apoptosis of A549 cells induced by rSIFN-co was also more significant than that of Infergen（P=0.000）.Cells treated with rSIFNco＋ Cisplatin has a higher apoptosis rate than those treated with rSIFN-co（P=0.004）or Cisplatin（P=0.023）.rSIFN-co increased the expression of Fas and decreased the expression of Bcl-2.Cells treated with rSIFN-co showed lower fluoroscence intensity of Bcl-2than those treated with Infergen（P 〈0.05）.Conclusion rSIFN-co inhibits the proliferation of A549 and its effect is stronger than that of Infergen.Cisplatin can further enhance the inhibiting effect of rSIFN-co.The inhibiting efficiency may be associated with the expression of apoptosis-related genes.

关 键 词：重组高效复合干扰素 肺腺癌 增殖 凋亡 

分 类 号：R734.2[医药卫生—肿瘤]