铜离子调控木质纤维素降解和糙皮侧耳形态发育的研究  

Influence of copper ion on lignocellulose degradation and morphological development of Pleurotus ostreatus

作　　者：杨雪薇[1] 张晓昱[1] 龚青[1] 马富英[1] 

机构地区：[1]华中科技大学生命科学与技术学院,湖北武汉430074

出　　处：《菌物学报》2015年第4期647-652,共6页Mycosystema

基　　金：国家自然科学基金(J1103514);国家重点基础研究发展计划(2014CB138301)

摘　　要：漆酶是一种含铜的多酚氧化酶,在木质素的降解中起重要作用。铜离子对漆酶的产生和活性有重要影响。通过向秸秆固体培养基中添加铜离子,研究铜离子对糙皮侧耳木质纤维素降解酶、木质纤维素降解和形态发育的影响。结果表明,添加铜离子能在一定程度上提高漆酶的活性,添加3mmol/L铜离子在第7天漆酶活性比对照高出71.2%,28d后添加铜离子的样品木质素降解率稍高出对照,添加铜离子能促进糙皮侧耳原基的分化和子实体的发育。Laccases are copper-containing polyphenol oxidases, which play an important role in lignin degradation. The production and activity of laccases are regulated by copper ion. The influences of copper on the lignocellulosic degradation and morphological development of Pleurotus ostrecltus cultivated in copper-supplemented corn stover substrate were evaluated. The results suggested that copper ion increased laccase activity, and the activity increased by 71.2% over the control under the addition of 3mmol/L Cu2＋ on day 7. Copper ion could also induce primordium differentiation and development and of fruiting body.

关 键 词：食用菌 金属离子 降解与转化 生长发育 

分 类 号：S646.141[农业科学—蔬菜学]