与草菇子实体形成和开伞相关的vv‐exp基因的克隆及表达分析  

Clone and expression analysis of a vv‐exp gene related to the formation and expansion of fruiting body in Volvariella volvacea

作　　者：卢园萍 连玲丹 郭丽(石羡)[1] 严俊杰[1] 谢斌[1] 王威[1] 谢宝贵[1] 

机构地区：[1]福建农林大学生命科学学院菌物研究中心,福建福州350002

出　　处：《菌物学报》2015年第4期717-723,共7页Mycosystema

基　　金：国家重点基础研究发展计划(2014CB138302);国家自然科学基金(31470107)

摘　　要：利用本地Blast,将灰盖鬼伞的高速泳动族蛋白(high-mobility-group box,HMGB)编码基因exp与草菇PYd21基因组进行比对,找到一个exp的同源基因vv-exp。克隆测序PYd21与PYd15两单孢菌株中vv-exp基因并进行比对,结果表明2个菌株的vv-exp基因序列一致。结合本实验室草菇转录组数据对vv-exp基因结构进行分析,结果显示此基因全长为1 937bp,共含3个内含子;开放阅读框长度为1 773bp,编码590个氨基酸。预测的氨基酸序列生物信息学分析结果表明,此基因编码的蛋白含有两个HMG-box结构域。通过实时荧光定量PCR(quantitative Real-time PCR,q RT-PCR)方法分析了vv-exp基因在草菇不同生长阶段以及不同部位的表达量,结果显示该基因在原基表达量较高,表明此基因可能与草菇子实体的形成有关;另外,在成熟期菌盖中vv-exp表达量比原基上调达到3倍以上,且显著高于伸长期菌盖,由此推断该基因参与调控草菇子实体开伞。Local BLAST analysis of comparing Coprinus cinereus expl with the genome of Volvariella volvacea strain PYd21 revealed a vv-exp gene homologue. Comparison between vv-exp gene of PYd21 and that of PYdlS indicated that the sequence was identical in both strains. The 1 937bp sequence contained a coding region of i 773bp interrupted by three different ntrons. Analysis of the deduced amino acid sequence demonstrated that the protein encoded by vv-exp contained two HMG-box domains. Expression of vv-exp was higher in the button stage than in vegetative mycelium, suggesting a role in fruiting body formation. Moreover, subsequent analysis showed that the expression of this gene in the pileus of the maturation stage was significantly higher than that in the pileus of the elongation stage, equaling 3 times higher than that in the primordium stage, implying that vv-exp might be involved in expansion of the pileus in V. volvacea in similar manner as in C. cinerea.

关 键 词：菌丝 原基 食用真菌 纽扣期 转录组 

分 类 号：S646.13[农业科学—蔬菜学]