脐带沃顿胶干细胞移植对脊髓损伤大鼠炎症因子表达的影响  

Effects of umbilical cord Wharton's jelly mesenchymal stem cell transplantation on the expression of inflammatory factors in rats with spinal cord injury

作　　者：马珊珊[1] 渠瑞娜 田毅[2] 姚宁[1] 崔渊博[1] 韩康[1] 邢衢 杨波[2] 关方霞[1,2] 

机构地区：[1]郑州大学生命科学学院,河南省郑州市450001 [2]郑州大学第一附属医院神经外科,河南省郑州市450052

出　　处：《中国组织工程研究》2015年第23期3729-3735,共7页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金资助项目(81171177;U1404313);河南省高校科技创新团队(15IRTSTHN022)~~

摘　　要：背景:免疫炎症反应促使大量炎症因子的产生和释放是继发性脊髓损伤的其对主要原因。目的:探讨脐带沃顿胶干细胞移植对急性脊髓损伤大鼠的神经修复作用及其对炎症因子单核细胞趋化蛋白1和白细胞介素10表达的影响。方法:81只健康成年雄性SD大鼠,随机分为假手术组、模型组和脐带沃顿胶干细胞移植组(n=27),后两组以脊髓半切方法建立急性脊髓损伤模型,造模后脐带沃顿胶干细胞移植组经尾静脉移植1×106个脐带沃顿胶干细胞。移植后不同时间通过BBB评分评价各组大鼠的运动功能;ELISA检测不同时间点各组大鼠血清中单核细胞趋化蛋白1的含量;qR T-PCR和Western分析不同时间点损伤脊髓组织中单核细胞趋化蛋白1及白细胞介素10的表达;免疫组织化学检测损伤组织中脐带沃顿胶干细胞的迁移和神经分化情况。结果与结论:与假手术组和模型组相比,脐带沃顿胶干细胞移植组大鼠的神经功能明显恢复(P<0.05)。脐带沃顿胶干细胞移植组大鼠血清中单核细胞趋化蛋白1水平显著低于模型组(P<0.05)。脐带沃顿胶干细胞移植组脊髓组织单核细胞趋化蛋白1 mR NA和蛋白表达显著低于模型组(P<0.05),白细胞介素10 mR NA和蛋白表达显著高于模型组(P<0.05)。移植组中脐带沃顿胶干细胞可迁移至损伤部位,并表达神经胶质纤维酸性蛋白。这些结果提示脐带沃顿胶干细胞可能通过调控脊髓损伤组织的炎症反应,促进神经修复,这也可能是脐带沃顿胶干细胞治疗脊髓损伤的机制之一。BACKGROUND： The production and release of a large amount of inflammatory factors caused by immune system inflammatory response mainly contributes to secondary spinal cord injury.OBJECTIVE： To investigate the effects of umbilical cord Wharton＇s jelly mesenchymal stem cell transplantation on repair of injured neurological function and expression of inflammatory factors monocyte chemoattractant protein 1and interleukin 10 in rats with acute spinal cord injury.METHODS： Eighty-one healthy adult male Sprague-Dawley rats were randomly and equally divided into sham operation,model and cell transplantation groups,with 27 rats per group. Rats in the latter two groups were subjected to hemisection of the spinal cord to establish acute spinal cord injury models. Rat models in the celltransplantation group received umbilical cord Wharton＇s jelly mesenchymal stem cell injection（1×106） via the tail vein.Rat neurological function was evaluated using the BBB score at different time points after spinal cord injury. The expression of monocyte chemoattractant protein 1 and interleukin 10 in injured spinal cord tissue was detected using ELISA assay at different time points after spinal cord injury. Migration and neuronal differentiation of umbilical cord Wharton＇s jelly mesenchymal stem cells in the injured spinal cord tissue were determined using immunohistochemical staining method.RESULTS AND CONCLUSION： Compared with the sham operation and model groups,rat neurological function was significantly recovered in the cell transplantation group（P〈0.05）. Compared to the model group,monocyte chemoattractant protein 1 level in the serum and monocyte chemoattractant protein 1 m RNA and protein expression in the injured spinal cord tissue were significantly lower（P〈0.05）,but interleukin 10 mR NA and protein expression in the injured spinal cord tissue was significantly higher（P〈0.05）,in the cell transplantation group. In the cell transplantation group,umbilical cord Wharton＇s jelly mesenchymal stem cell

关 键 词：脊髓损伤 脐带 干细胞移植 白细胞介素10 干细胞 移植 沃顿胶干细胞 神经修复 单核细胞趋化蛋白1 国家自然科学基金 

分 类 号：R394.2[医药卫生—医学遗传学]