NGX6基因联合顺铂治疗肺癌的体内外研究  

NGX6 gene combined with cisplatin in treatment of lung cancer in vitro and in vivo

作　　者：张敬[1] 王哲[2] 史晓宇[1] 孟玮[1] 马峰[1] 王金[3] 赵永明[3] 

机构地区：[1]河北北方学院附属第一医院肿瘤内科,河北张家口075000 [2]河北北方学院附属第一医院泌尿外科,河北张家口075000 [3]河北北方学院药学系,河北张家口075000

出　　处：《中国生化药物杂志》2015年第5期33-36,共4页Chinese Journal of Biochemical Pharmaceutics

基　　金：张家口市2013年度科学技术研究与发展计划(1321099D)

摘　　要：目的探讨NGX6基因联合顺铂对肺癌A549细胞和NCI-H1975细胞的体外抑制作用及其体内抗肿瘤效果。方法以脂质体鱼精蛋白为载体,构建载NGX6基因的阳离子脂质体-DNA复合物。将A549细胞和NCI-H1975细胞均分为NGX6组(载NGX6基因的LPD,NGX6浓度为30μg/m L)、顺铂组、NGX6+顺铂组,以PBS为阴性对照。采用MTT检测各组对A549细胞和NCI-H1975细胞的生长抑制作用;克隆形成实验计算克隆形成率和抑制率;构建肺癌移植肿瘤模型,将裸鼠分为同细胞处理相同的4组,每组10只,统计各组裸鼠肿瘤体积和生存期,观察各组裸鼠肿瘤组织细胞凋亡情况。结果对肿瘤细胞的增殖抑制能力显著强于NGX6组和顺铂(P<0.01)。克隆形成实验结果表明,NGX6+顺铂组的肿瘤细胞克隆数小于NGX6组和顺铂组(P<0.01)。体内抗肿瘤实验结果表明,NGX6+顺铂组对荷瘤裸鼠的肿瘤生长抑制率显著高于NGX6组和顺铂组(P<0.01)。NGX6+顺铂组,NGX6组,顺铂组和生理盐水组荷瘤裸鼠的中位生存期分别为43、31、29、15d。结论 NGX6基因联合顺铂能够有效抑制肺癌细胞的增殖和肿瘤的生长。表明基因干预联合细胞毒性药物化疗能为肿瘤的治疗提供了一种新的治疗手段。Objective To evaluate the effect of NGX6 combined with cisplatin on the inhibition rate of A549 cells and NCI-H1975 cells and antitumor effects in vivo.Methods The NGX6 was loaded in the LPD, and prepare Liposome protamine DNA complexes.A549 cells and NCI-H1975 cells were seperately divided into NGX6 group （ 30 μg/mL NGX6 concentration ） , cisplatin group, NGX6 ＋cisplatin group, PBS as negative control group.The effect of cytotoxicity of four group on A549 cells and NCI-H1975 cell were evaluated by MTT assay.the clone forming rate and the inhibition rate were determined by Cell colony count.lung transplantation tumor model were successfully established, then nude mice were divided into four groups as abeve, each group of 10, tumor size and survival period were determined tumor cell apoptosis were observed.Results The cell viability of A549 cells and NCI-H1975 cells of （NGX6 ＋cisplatin） were lower than that of NGX6 group, cisplatin group and saline group, respectively（P〈0.01）.The cloning efficiency of A549 cells and NCI-H1975 cells of （ NGX6 ＋cisplatin） were lower than that of NGX6 group, cisplatin group and saline group, respectively（P〈0.01）.The tumor inhibitory rate was in vivo for （NGX6 ＋cisplatin） was higher than other group（P〈0.01）.The median survival of nude mice in （NGX6 ＋cisplatin）, NGX6, cisplatin and saline group were 43,31,29 and 15 days.Conclusion NGX6 combination with cisplatin can inhibit the cell proliferate of lung cancer cells and inhibit the tumor growth and the combination of NGX6 and cisplatin may be a potentially effective treatment for lung cancer.

关 键 词：NGX6基因 肺癌 顺铂 A549细胞 NCI-H1975细胞 体内 体外 

分 类 号：R734.2[医药卫生—肿瘤]