程序性死亡-1对骨肉瘤细胞MG-63的影响研究  

Effect of programmed death-1 on human osteosarcoma cells MG-63

作　　者：钱茜[1] 包德明 岳宗进[3] 

机构地区：[1]河南中医学院第一附属医院药学部,河南郑州450000 [2]郑州大学第一附属医院骨科,河南郑州450052 [3]河南省中医院脊柱研究所,河南郑州450002

出　　处：《中国生化药物杂志》2015年第5期40-43,共4页Chinese Journal of Biochemical Pharmaceutics

基　　金：河南省科技攻关计划(122102310186)

摘　　要：目的探讨程序性死亡-1(programmed death-1,PD-1)对骨肉瘤细胞MG-63的影响。方法从人骨肉瘤细胞株MG-63细胞中分选及鉴定骨肉瘤类肿瘤干细胞,MTT法检测PD-1信号对T细胞增殖的影响;RT-PCR检测PD-1 mRNA表达。结果 1周内癌细胞明显增殖,显示出较强的增殖能力及侵袭性;肿瘤细胞球的形成依赖于血清营养的支持;血清培养基中的MG-63癌细胞增殖数量较无血清悬浮培养的骨肉瘤细胞球细胞明显增高(P<0.05);RT-PCR的结果显示多能干细胞标记CD133在癌细胞球中的表达显著高于MG-63,癌细胞球及MG-63的PD-1表达均明显增加(P<0.05)。结论 MG-63细胞系具有骨肉瘤干细胞特性,能够表达相应的细胞标记,PD-1的表达亦显著增加,其使患者的免疫功能下调,与肿瘤的发生及进展密切相关。Objective To discuss the influences of programmed death-1 （PD-1） factor on osteosarcoma cells MG-63.Methods Osteosarcoma stem cells were sovted and identified through osteosarcoma cell strain MG-63 cells.The influence of PD-1 signal on T cells proliferation were detected by MTT. The expression of PD-1 mRNA was detected by RT-PCR.Results Cancer cells had an obvious proliferation within one week, which showed the strong ability of proliferation and aggressivity.The formation of tumor cells spheres depended on the support of serum nutrition.The number of MG-63 cells proliferation in serum culture medium was significantly higher than the osteosarcoma cells spheres in serum-free suspension culture （ P〈0.05 ） .Pluripotent stem cell marks that the expression of CD133 in cancer cell sphere was significantly higher than that of MG-63（P〈0.05）.RT-PCR results showed the PD-1 expression level of cancer cell sphere and MG-63 were increased significantly.Conclusion MG-63 cell line has the character istics of osteosarcoma stem cells.MG-63 cell line can express the corresponding cell markers.The expression of PD-1 also increase significantly which can reduce immune function of patients and is closely related with the occurrence and development of tumors.

关 键 词：PD-1 MG-63 骨肉瘤 干细胞 

分 类 号：R738.7[医药卫生—肿瘤]