白色念珠菌对人口腔黏膜上皮角质细胞的黏附作用及影响因素研究  

Study on the adhesion of Candida albicans to human oral epithelial keratinocytes and the affecting factors

作　　者：孙富丽[1] 张英[1] 闻妍[1] 

机构地区：[1]中国医科大学口腔医学院,辽宁省口腔医学研究所口腔黏膜病学教研室,辽宁省口腔疾病转化医学中心,沈阳110002

出　　处：《中国实用口腔科杂志》2015年第6期350-354,共5页Chinese Journal of Practical Stomatology

基　　金：辽宁省自然科学基金(20092115)

摘　　要：目的 研究白色念珠菌对人口腔黏膜上皮角质细胞（human oral keratinocytes,HOK）的黏附作用及影响因素.方法 本研究于2012年8月至2014年3月在中国医科大学附属口腔医院中心实验室完成.体外培养白色念珠菌和HOK,在不同浓度、温度和pH值条件下,用白色念珠菌浊悬液感染HOK,并进行革兰染色,油镜下观察,计算黏附指数、黏附率.结果 在同一浓度下,菌丝相的白色念珠菌黏附率明显高于孢子相的;随着白色念珠菌浓度的升高,黏附率也有所增加.另外,白色念珠菌浓度为107 CFU/mL与HOK的细胞浓度为105个/mL时黏附基本达到饱和,可作为后续试验的最佳浓度.随着温度升高,白色念珠菌的黏附率也随之增加.黏附的最适宜pH值范围是6.0～8.O.结论 白色念珠菌黏附率的增加与菌丝的形成有关,有利于菌丝形成和生长的条件均可提高黏附率.Objective To study the adhesion of Candida albieans to human oral epithelial keratinocytes and the affect-ing factors. Methods The first step was the cultivation of candida albicans standards strains and human oral epithelialkeratinocytes, then different phenotypes of Candida albicans were co-cultured with human oral epithelial keratinoeytesat different concentration, temperature and pH, and last, gram＇s staining was made; observe the adhesion test result un-der oil microscope and calculate adhesion index and adhesion rate. Results The adhesion rate of hyphae Candida albi-cans was much higher than spore （P 〈 0.05 ） ; as the concentration of Candida albicans increased, adhesion rate increasedas well; adhesion rate was highest when pH was 6.0 to 8.0. Adhesion rate of 37℃ was higher than that of 25℃（P 〈0.05）. Conclution Increased adhesion rate of Candida albicans is associated with the formation of hyphae ; the condi-tions which help the formation and growth of hyphae would increase the adhesion rate.

关 键 词：白色念珠菌 人口腔黏膜上皮角质细胞 黏附 

分 类 号：R78[医药卫生—口腔医学]