Propachlor 对前列腺癌细胞 PC3及 LNCaP 的作用及其可能机制  

The function and possible mechanism of Propachlor on anti-prostate cancer LNCaP and PC3 cells

作　　者：邰胜[1,2] 徐凌凡[1] 徐明[1] 张阳阳[1] 张力[1,2] 梁朝朝[1,2] 

机构地区：[1]安徽医科大学第一附属医院泌尿外科,合肥230022 [2]安徽医科大学泌尿外科研究所,合肥230032

出　　处：《安徽医科大学学报》2015年第8期1045-1048,共4页Acta Universitatis Medicinalis Anhui

基　　金：安徽省自然科学基金(编号:1308085QH132);国家自然科学基金青年科学基金培养计划(编号:2012KJ13);国家临床重点专科建设项目基金(编号:2100299);院内博士科研基金(编号:20120119)

摘　　要：目的探讨化合物Propachlor对前列腺癌的作用及可能的分子机制。方法用梯度浓度的化合物Propachlor处理前列腺癌PC3及LNCa P细胞株;使用Cell Titer方法检测肿瘤细胞的存活率,Western blot法检测细胞自噬蛋白LC3-Ⅱ的转变情况,荧光显微镜检测细胞中诱导LC3质粒标记绿色荧光蛋白(GFP-LC3)的表达情况。结果 Propachlor可以抑制前列腺癌PC3及LNCa P细胞的存活率,同时剂量越大肿瘤细胞存活率抑制越明显;Propachlor可以诱导PC3及LNCa P肿瘤细胞自噬蛋白LC3-Ⅱ及自噬小体GFP-LC3的表达,诱导自噬效应同Propachlor的剂量正相关。结论化合物Propachlor具有抗前列腺癌的生物学效应,同时该抗肿瘤效应是通过激活细胞内自噬效应产生的。Objective To explore the mechanism of Propachlor anti-prostate cancer. Methods The LNCaP and PC3 cells were treated with Propachlor with the gradient concentration. The CellTiter was performed to test the cell variability, and the western blot was also performed to detect the LC3-Ⅱ conversion. The GFP-LC3 was observed under the fluorescence microscope. Results The PC3 and LNCaP cells variability was inhibited with the Propa-chlor, and little cell variability was linked with high concentration Propachlor. The Propachlor might induce the PC3 and LNCaP cells autophagy with LC3-Ⅱ conversion and GFP-LC3 dots increase, which was also linked with high concentration Propachlor. Conclusion The compound Propachlor may be anti-prostate cancer via induction of autophagy among prostate cancer cells.

关 键 词：前列腺癌 Propachlor 自噬 

分 类 号：R737.25[医药卫生—肿瘤]