小鼠S180移植瘤毛细淋巴管超微结构随肿瘤生长的变化  

Ultrastructure of lymphatic capillaries with tumor growth in Kunming mice transplanted with S180 sarcoma

作　　者：马楠[1] 张丽男[1] 王龙凯 张晓博[1] 毕秀[1] 高子彬[1] 

机构地区：[1]河北科技大学药学系,石家庄050018

出　　处：《解剖学报》2015年第4期503-508,共6页Acta Anatomica Sinica

基　　金：国家自然科学基金资助项目(81402886);河北省杰出青年科学基金资助项目(H2014208004)

摘　　要：目的观察小鼠肉瘤(S180)移植瘤发展过程中毛细淋巴管超微结构,探究随着荷瘤时间的延长,移植瘤内毛细淋巴管超微结构的变化。方法腋下注射S180腹水型瘤株,建立S180荷瘤小鼠肉瘤模型,共15只,每组3只;采用透射电子显微镜技术观察小鼠S180移植瘤组织毛细淋巴管的超微结构,分析肿瘤组织毛细淋巴管超微结构随肿瘤生长的变化情况。结果 3d组标本观察发现,毛细淋巴管管壁结构完整,管壁可见锚丝与胶原纤维,毛细淋巴管内皮细胞胞质内可见各种结构正常的细胞器;5d组、7d组及9d组观察到随着荷瘤时间的延长,毛细淋巴管管壁断裂溶解情况越来越严重,可观察到的锚丝与胶原纤维数量越来越少,毛细淋巴管内皮细胞胞质内各种细胞器受损程度也越来越严重;11d组观察到毛细淋巴管管壁几乎全部破碎溶解,未观察到锚丝与胶原纤维,无法清晰辨认淋巴内皮细胞,未观察到细胞器。结论随着荷瘤时间的延长,肿瘤体积和重量呈增长趋势,毛细淋巴管管壁受损程度、淋巴内皮细胞胞质内细胞器的受损程度逐渐加重。Objective To investigate and explore the ultrastructural change of lymphatic capillaries in Kunming mice transplanted with S180 sarcoma with tumor growth. Methods Fifteen Kunming mice were inoculated with S180 ascites via subcutaneous injection to establish the cancer model and divided into five groups. The ultrastructural features of lymphatic capillaries of S180 sarcoma transplanted in Kunming mice were observed by a transmission electron microscope（ TEM） in order to analyze the ultrastructural change of lymphatic capillaries with tumor growth. Results The integral lymphatic capillary wall,the anchoring filament and collagen fiber,and the organelles with normal physiological structure were observed in 3 days group. The dissolved or damaged degree of the lymphatic capillary wall and organelles became more and more serious,and the number of the anchoring filament and collagen became less and less with tumor growth in 5 days group,7 days group and 9 days group. The lymphatic capillary wall was completely destroyed or broken,and any anchoring filament and collagen fiber were scarcely observed,while the lymphatic capillary endothelial cells and the organelles were scarcely observed or identified in 11 day group. Conclusion With the development of transplanted S180,the tumor volume and weight were increased and the dissolved or damaged degree of the lymphatic capillary wall and the organelles became progressively worse.

关 键 词：S180移植瘤 毛细淋巴管 超微结构 透射电子显微镜 小鼠 

分 类 号：R73[医药卫生—肿瘤]