孤雌胚胎干细胞来源的诱导多能干细胞的建立及对印记基因表达的影响  

Generation of induced pluripotent stem cells derived from parthenogenetic embryonic stem cells and reprogramming of imprinted genes

作　　者：单智焱[1] 武玢 张玥[1] 薛媛[1] 吴嫣爽[1] 沈星辉[1] 雷蕾[1] 刘忠华[2] 

机构地区：[1]哈尔滨医科大学组织学与胚胎学教研室,哈尔滨150081 [2]东北农业大学生命科学院,哈尔滨150081

出　　处：《解剖学报》2015年第4期553-557,共5页Acta Anatomica Sinica

基　　金：国家自然科学基金资助项目(C120115)

摘　　要：目的通过诱导多能干细胞(i PSCs)技术重编程孤雌胚胎干细胞,探讨i PSCs技术对孤雌胚胎干细胞的多能性及印记基因的影响。方法从孤雌激活的囊胚中建立了孤雌胚胎干细胞;利用反转录病毒将多能性转录因子转入孤雌胚胎干细胞中,建立孤雌i PS细胞。结果建立的孤雌来源的i PS细胞体内外分化能力与孤雌胚胎干细胞的差别无显著性;Real-time PCR结果显示,孤雌i PS细胞母源印记基因的表达明显高于孤雌胚胎干细胞,父源印记基因表达下降,多能性基因表达升高。结论 i PSCs技术能影响基因的表达,尤其是印记基因,印记使其更接近于正常受精来源的胚胎干细胞中印记基因水平。Objective To investigate the effect of pluripotency and the expression of imprinted genes on the parthenogenetic embryonic stem cells（ Pg ESCs） by induced pluripotent stem cells（ i PSCs） reprogrmming. Methods In our study,we firstly generated the Pg ESCs from parthenogenetic blastocyst,induced the pluripotent factors into the Pg ESCs by retrovirus transfection and generated the i PS cells derived from Pg ESCs. Results It showed no difference in pluripotency and differentiation between Pg ESCs and Pg ESC-i PS cells,however,the expression of maternal imprinted genes was significantly increased and the expression of paternal imprinted genes was decreased in Pg ESC-i PS cells compared to that in Pg ESCs. Conclusion These results demonstrated the expression of imprinted genes could be modified by i PSCs reprogramming,which may be close to the expression level of imprinted genes in fertilized embronic stem cells.

关 键 词：孤雌胚胎干细胞 诱导多能干细胞 印记基因 实时定量聚合酶链反应 小鼠 

分 类 号：R329.1[医药卫生—人体解剖和组织胚胎学]