栓皮栎胚性和非胚性愈伤组织生化特性研究  

Biochemical Characteristic Variations of Embryogenic and Non-embryogenic Callus of Quercus variabilis

作　　者：孟姣[1] 张存旭[1] 孙琳[1] 戎旭旭 

机构地区：[1]西北农林科技大学林学院,陕西杨陵712100

出　　处：《西北林学院学报》2015年第5期106-110,共5页Journal of Northwest Forestry University

基　　金：西北农林科技大学唐仲英育种专项

摘　　要：植物体细胞胚胎发生是一个复杂的过程,为进一步揭示胚性愈伤组织诱导的潜在能力,以栓皮栎未成熟合子胚诱导产生的胚性愈伤组织和非胚性愈伤组织为材料,通过对可溶性糖、淀粉、可溶性蛋白、游离脯氨酸、总酚含量和蛋白酶活性的测定,研究体细胞胚胎发生过程中2种愈伤组织生化特性的差异。结果表明,胚性愈伤组织中可溶性糖、淀粉、可溶性蛋白质及脯氨酸含量分别为97.6、25.3、28.3mg·g-1和202.8mg·g-1,均高于非胚性愈伤组织;两者的蛋白酶活性无显著性差异;但非胚性愈伤组织中多酚类化合物的含量显著高于胚性愈伤组织。Somatic embryogenesis is a complex process. In order to reveal the regeneration potential of embryogenic callus, this paper investigated the difference of some biochemical characteristics, including soluble sugars, soluble proteins, starch, free proline, phenolics and proteolytic activity in embryogenic and non-embryogenic callus induced by immature zygotic embryos of Quercus variabilis. The contents of soluble sugars, soluble proteins, starch, free proline in embryogenic callus were 97.6, 25.3, 28.3, and 202.8 mg·g-1, respectively, more than those in non-embryogenic callus; while proteolytic activity in two calli had no significant difference. However, non-embryogenic callus had a higher phenolics level compared with embryogenic callus.

关 键 词：体细胞胚胎发生 胚性能力 碳水化合物 蛋白质 多酚类物质 

分 类 号：S792.18[农业科学—林木遗传育种]